Pichia acaciae Killer System: Genetic Analysis of Toxin Immunity

• Published in: Applied and Environmental Microbiology Vol. 73; no. 13; pp. 4373 - 4378
• Main Authors: Paluszynski, John P, Klassen, Roland, Meinhardt, Friedhelm
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.07.2007
• Subjects: Base Sequence; Biological and medical sciences; Cells; DNA Primers - genetics; DNA, Fungal - genetics; Fundamental and applied biological sciences. Psychology; Gene expression; Genes, Fungal; genetic techniques and protocols; Genetics and Molecular Biology; immunity; Immunity (Disease); Killer Factors, Yeast; Microbiology; Mycotoxins - genetics; Pichia; Pichia - genetics; Plasmids - genetics; Saccharomyces cerevisiae; Saccharomyces cerevisiae - genetics; Toxins; Yeast; Fungi; Toxin; Yeast; Genetics; Ascomycetes; Immunity; Thallophyta; Pichia

The gene responsible for self-protection in the Pichia acaciae killer plasmid system was identified by heterologous expression in Saccharomyces cerevisiae. Resistance profiling and conditional toxin/immunity coexpression analysis revealed dose-independent protection by pPac1-2 ORF4 and intracellular...