Pichia acaciae Killer System: Genetic Analysis of Toxin Immunity

The gene responsible for self-protection in the Pichia acaciae killer plasmid system was identified by heterologous expression in Saccharomyces cerevisiae. Resistance profiling and conditional toxin/immunity coexpression analysis revealed dose-independent protection by pPac1-2 ORF4 and intracellular...

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Published inApplied and Environmental Microbiology Vol. 73; no. 13; pp. 4373 - 4378
Main Authors Paluszynski, John P, Klassen, Roland, Meinhardt, Friedhelm
Format Journal Article
LanguageEnglish
Published Washington, DC American Society for Microbiology 01.07.2007
Subjects
Base Sequence
Biological and medical sciences
Cells
DNA Primers - genetics
DNA, Fungal - genetics
Fundamental and applied biological sciences. Psychology
Gene expression
Genes, Fungal
genetic techniques and protocols
Genetics and Molecular Biology
immunity
Immunity (Disease)
Killer Factors, Yeast
Microbiology
Mycotoxins - genetics
Pichia
Pichia - genetics
Plasmids - genetics
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Toxins
Yeast
Fungi
Toxin
Yeast
Genetics
Ascomycetes
Immunity
Thallophyta
Pichia
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Summary:The gene responsible for self-protection in the Pichia acaciae killer plasmid system was identified by heterologous expression in Saccharomyces cerevisiae. Resistance profiling and conditional toxin/immunity coexpression analysis revealed dose-independent protection by pPac1-2 ORF4 and intracellular interference with toxin function, suggesting toxin reinternalization in immune killer cells.
Bibliography:http://aem.asm.org/contents-by-date.0.shtml
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Corresponding author. Mailing address: Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität Münster, Corrensstr. 3, D-48149 Münster, Germany. Phone: 49 2 51 83-3 98 25. Fax: 49 2 51 83-3 83 88. E-mail: meinhar@uni-muenster.de
ISSN:0099-2240
1098-5336
DOI:10.1128/AEM.00271-07