Anticancer effect of SZC017, a novel derivative of oleanolic acid, on human gastric cancer cells

• Published in: Oncology reports Vol. 35; no. 2; pp. 1101 - 1108
• Main Authors: GAO, LEI, XU, ZHEN, WANG, YAN, SUN, BIN, SONG, ZHICHENG, YANG, BINING, LIU, XU, LIN, YUAN, PENG, JINYONG, HAN, GUOZHU, WANG, SHISHENG, TANG, ZEYAO
• Format: Journal Article
• Language: English
• Published: Greece D.A. Spandidos 01.02.2016; Spandidos Publications; Spandidos Publications UK Ltd
• Subjects: Antineoplastic Agents - chemical synthesis; Antineoplastic Agents - pharmacology; Apoptosis; Apoptosis - drug effects; Care and treatment; Cell cycle; Cell Cycle - drug effects; Cell Line, Tumor; Cellular signal transduction; Chromatography; Cytoplasm; Deoxyribonucleic acid; DNA; DNA Topoisomerases, Type I - biosynthesis; DNA Topoisomerases, Type I - genetics; Drug Screening Assays, Antitumor; Gastric cancer; Gene expression; Health aspects; Humans; I-kappa B Proteins - biosynthesis; I-kappa B Proteins - genetics; MGC-803; Neoplasm Proteins - antagonists & inhibitors; Neoplasm Proteins - biosynthesis; Neoplasm Proteins - genetics; NF-kappa B - antagonists & inhibitors; NF-KappaB Inhibitor alpha; Oleanolic Acid - analogs & derivatives; Oleanolic Acid - chemical synthesis; Oleanolic Acid - pharmacology; oleanolic acid derivative; Penicillin; Phosphorylation; Piperidines - chemical synthesis; Piperidines - pharmacology; Proto-Oncogene Proteins c-akt - biosynthesis; Proto-Oncogene Proteins c-akt - genetics; SGC-7901; Signal Transduction - drug effects; Stomach cancer; Stomach Neoplasms - pathology; SZC017; Terpenes; Topoisomerase Inhibitors - chemical synthesis; Topoisomerase Inhibitors - pharmacology; United States > US; China
• ISSN: 1021-335X; 1791-2431
• DOI: 10.3892/or.2015.4447

Oleanolic acid (OA) and its several derivatives possess chemopreventive and chemotherapeutic functions against a series of cancer types. Many chemotherapeutic compounds are effective in improving the quality of life and prolonging the survival of patients with gastric cancer, therefore progress in the treatment of gastric cancer, especially the anticancer effects of OA derivatives must be achieved. The inhibitory effect of SZC017, a newly synthesized derivative of OA, on cell viability was determined by MTT assay. Furthermore, flow cytometry, transmission electron microscopy, and western blot analysis revealed that the inhibition of cell viability by OA was mediated by triggering the intrinsic apoptosis of gastric cancer cells, and inducing S phase arrest of SGC7901 cells. Mechanistically, SZC017 was effective against gastric cancer cells via inhibiting Akt/NF-κB signaling and topoisomerase I and IIα proteins. Taken together, our data indicate that SZC017 may be a potential chemotherapeutic agent against gastric cancer cells.