Reduction of the infectivity of Toxoplasma gondii and Eimeria stiedai sporozoites by treatment with bovine lactoferricin

• Published in: Journal of Veterinary Medical Science Vol. 63; no. 2; pp. 187 - 190
• Main Authors: Omata, Y. (Obihiro Univ. of Agriculture and Veterinary Medicine, Hokkaido (Japan)), Satake, M, Maeda, R, Saito, A, Shimazaki, K, Yamauchi, K, Uzuka, Y, Tanabe, S, Sarashina, T, Mikami, T
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.02.2001; Japan Science and Technology Agency
• Subjects: Animals; Antiprotozoal Agents - pharmacology; Cells, Cultured; Coccidiosis - drug therapy; Coccidiosis - parasitology; Coccidiosis - prevention & control; EIMERIA; Eimeria - drug effects; Eimeria - growth & development; Eimeria - pathogenicity; Eimeria stiedai; lactoferricin; Lactoferrin - analogs & derivatives; Lactoferrin - pharmacology; MICE; MILK PROTEIN; Parasite Egg Count; PATHOGENICITY; RABBITS; Toxoplasma - drug effects; Toxoplasma - growth & development; Toxoplasma - pathogenicity; TOXOPLASMA GONDII; Toxoplasmosis - drug therapy; Toxoplasmosis - parasitology; Toxoplasmosis - prevention & control
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.63.187

Sporozoites of Toxoplasma gondii preincubated with lactoferricin showed decreased activity in penetration of mouse embryonal cells. Mice inoculated with 105 sporozoites preincubated with lactoferricin showed a higher survival rate than those inoculated with the same number of untreated sporozoites. Likewise, sporozoites of Eimeria stiedai preincubated with lactoferricin also showed decreased activity in penetration of rabbit hepatobiliary cells. Rabbits inoculated with 105 sporozoites preincubated with lactoferricin shed fewer oocysts than those inoculated with the same number of untreated sporozoites. These results indicate that lactoferricin is effective to reduce the infectivity of sporozoites of Toxoplasma gondii and Eimeria stiedai.