Effects of Seed Bio-Priming by Purple Non-Sulfur Bacteria (PNSB) on the Root Development of Rice

• Published in: Microorganisms (Basel) Vol. 10; no. 11; p. 2197
• Main Authors: Iwai, Ranko, Uchida, Shunta, Yamaguchi, Sayaka, Sonoda, Fumika, Tsunoda, Kana, Nagata, Hiroto, Nagata, Daiki, Koga, Aoi, Goto, Midori, Maki, Taka-aki, Hayashi, Shuhei, Yamamoto, Shinjiro, Miyasaka, Hitoshi
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 01.11.2022; MDPI
• Subjects: Agricultural production; Agriculture; Bacteria; bio-priming; Cell culture; Cultivars; Development; Environmental aspects; Experiments; Field study; Grain; Image analysis; Image processing; Laboratories; Light; lipopolysaccharide (LPS); Lipopolysaccharides; Physiological aspects; Plant growth; Plants; Priming; Proteobacteria; purple non-sulfur bacteria; Rhodobacter sp; Rhodopseudomonas sp; Rice; Root development; Roots (Botany); Seeds; Strains (organisms); Sulfur; Sulfur bacteria; Japan; Canada
• ISSN: 2076-2607; 2076-2607
• DOI: 10.3390/microorganisms10112197

The effects of seed bio-priming (seed soaking) with purple non-sulfur bacteria (PNSB) on the grain productivity and root development of rice were examined by a field study and laboratory experiments, respectively. Two PNSB strains, Rhodopseudomonas sp. Tsuru2 and Rhodobacter sp. Tsuru3, isolated from the paddy field of the study site were used for seed bio-priming. For seed bio-priming in the field study, the rice seeds were soaked for 1 day in water containing a 1 × 105 colony forming unit (cfu)/mL of PNSB cells, and the rice grain productivities at the harvest time were 420, 462 and 504 kg/are for the control, strain Tsuru2-primed, and strain Tsuru3-primed seeds, respectively. The effects of seed priming on the root development were examined with cell pot cultivation experiments for 2 weeks. The total root length, root surface area, number of tips and forks were evaluated with WinRhizo, an image analysis system, and strains Tsuru2- and Tsuru3-primed seeds showed better root development than the control seeds. The effects of seed priming with the dead (killed) PNSB cells were also examined, and the seed priming with the dead cells was also effective, indicating that the effects were attributed to some cellular components. We expected the lipopolysaccharide (LPS) of PNSB as the effective component of PNSB and found that seed priming with LPS of Rhodobacter sphaeroides NBRC 12203 (type culture) at the concentrations of 5 ng/mL and 50 ng/mL enhanced the root development.