Blackberry-Loaded AgNPs Attenuate Hepatic Ischemia/Reperfusion Injury via PI3K/Akt/mTOR Pathway

• Published in: Metabolites Vol. 13; no. 3; p. 419
• Main Authors: Fathi, Ahmed M, Waz, Shaimaa, Alaaeldin, Eman, Toni, Nisreen D M, El-Sheikh, Azza A K, Sayed, Ahmed M, Abdelmohsen, Usama Ramadan, Nazmy, Maiiada H
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.03.2023; MDPI
• Subjects: 1-Phosphatidylinositol 3-kinase; Adenosine triphosphate; AgNPs; AKT protein; Analysis; Anthocyanins; Antibodies; Antioxidants; Apoptosis; Bax protein; Berries; blackberry; caspase-3; cell apoptosis; Diagnosis; Flavonoids; Glucosides; Inflammation; Ischemia; Liver; liver ischemia-reperfusion injury; Metabolism; Metabolites; Metabolomics; Molecular dynamics; Molecular modelling; Nanoparticles; Natural products; Oxidative stress; Phosphatase; Phospholipase A2; Phosphorylation; PI3K/Akt/mTOR; Proteins; Protocol; Reperfusion; Reperfusion injury; Risk factors; Signal transduction; Silver; TOR protein; Egypt; Cairo Egypt; United Kingdom > UK; United States > US; Germany; blackberry; metabolomics; AgNPs; cell apoptosis; liver ischemia-reperfusion injury; PI3K/Akt/mTOR; caspase-3
• ISSN: 2218-1989; 2218-1989
• DOI: 10.3390/metabo13030419

Liver ischemia-reperfusion injury (IRI) is a pathophysiological insult that often occurs during liver surgery. Blackberry leaves are known for their anti-inflammatory and antioxidant activities. To achieve site-specific delivery of blackberry leaves extract ( ) loaded AgNPs to the hepatocyte in IRI and to verify possible molecular mechanisms. IRI was induced in male Wister rats. Liver injury, hepatic histology, oxidative stress markers, hepatic expression of apoptosis-related proteins were evaluated. Non-targeted metabolomics for chemical characterization of blackberry leaves extract was performed. Pre-treatment with protected against the deterioration caused by I/R, depicted by a significant improvement of liver functions and structure, as well as reduction of oxidative stress with a concomitant increase in antioxidants. Additionally, promoted phosphorylation of antiapoptotic proteins; PI3K, Akt and mTOR, while apoptotic proteins; Bax, Casp-9 and cleaved Casp-3 expressions were decreased. LC-HRMS-based metabolomics identified a range of metabolites, mainly flavonoids and anthocyanins. Upon comprehensive virtual screening and molecular dynamics simulation, the major annotated anthocyanins, cyanidin and pelargonidin glucosides, were suggested to act as PLA2 inhibitors. can ameliorate hepatic IRI augmented by -AgNPs nano-formulation via suppressing, oxidative stress and apoptosis as well as stimulation of PI3K/Akt/mTOR signaling pathway.