Genetic and Phenotypic Variability among Salmonella enterica Serovar Typhimurium Isolates from California Dairy Cattle and Humans

• Published in: Applied and Environmental Microbiology Vol. 72; no. 10; pp. 6632 - 6637
• Main Authors: Adaska, J.M, Silva, A.J, Berge, A.C.B, Sischo, W.M
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.10.2006
• Subjects: Animals; Anti-Bacterial Agents; Anti-Bacterial Agents - pharmacology; Antibiotic resistance; antimicrobial resistance patterns; Bacterial Typing Techniques; bacteriophages; Bacteriophages - genetics; Biological and medical sciences; California; Cattle; Dairy cattle; DNA; DNA fingerprinting; drug effects; drug resistance; Drug Resistance, Bacterial; Drug Resistance, Bacterial - physiology; Electrophoresis, Gel, Pulsed-Field; Fundamental and applied biological sciences. Psychology; Genetic testing; Genetic Variation; Genetics; Genotype & phenotype; human diseases; Human subjects; Humans; infection; insertion sequence typing; isolation; isolation & purification; microbial genetics; Microbiology; phage typing; pharmacology; physiology; plasmid typing; plasmids; Plasmids - genetics; Public Health Microbiology; pulsed-field gel electrophoresis; ribotypes; Ribotyping; Salmonella enterica; Salmonella enterica - genetics; Salmonella enterica subsp. enterica serovar Typhimurium; Salmonella Infections; Salmonella Infections - microbiology; Salmonella Typhimurium; Salmonella typhimurium - drug effects; Salmonella typhimurium - genetics; Salmonella typhimurium - isolation & purification; salmonellosis; strains; Studies; transposons; California; United States > US; Human; Vertebrata; Mammalia; Genetic variability; Dairy cattle; Bacteria; Isolate; Artiodactyla; Salmonella typhimurium; Ungulata; Enterobacteriaceae
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/AEM.01038-06

Fifty-six human and 24 adult dairy cattle isolates of Salmonella enterica serovar Typhimurium from a single county in California were compared using ribotyping, insertion sequence typing (IS200), pulsed-field gel electrophoresis, plasmid typing, phage typing, and antimicrobial resistance testing. The majority of the isolates fell into one of two groups which were phage types DT104 and DT193. Combining the information from all typing methods, a total of 45 different "clusters" were defined, with 35 of those including only a single isolate. The library of isolates had a high degree of variability, but antibiotic resistance and plasmid typing each defined single clusters in which human or bovine isolates predominated (χ2, P < 0.05).