ArtinM, a d -mannose-binding lectin from Artocarpus integrifolia , plays a potent adjuvant and immunostimulatory role in immunization against Neospora caninum

• Published in: Vaccine Vol. 29; no. 49; pp. 9183 - 9193
• Main Authors: Cardoso, Mariana R.D, Mota, Caroline M, Ribeiro, Dâmaso P, Santiago, Fernanda M, Carvalho, Julianne V, Araujo, Ester C.B, Silva, Neide M, Mineo, Tiago W.P, Roque-Barreira, Maria C, Mineo, José R, Silva, Deise A.O
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 15.11.2011; Elsevier
• Subjects: Adjuvants, Immunologic - pharmacology; Allergy and Immunology; Animals; Antibodies, Protozoan - blood; Antibodies, Protozoan - immunology; antigens; Apicomplexa; Applied microbiology; ArtinM; Artocarpus - chemistry; Artocarpus heterophyllus; Artocarpus integrifolia; Biological and medical sciences; brain; Brain - parasitology; cattle; Coccidiosis - immunology; Coccidiosis - prevention & control; cytokines; Cytokines - immunology; disease control; dogs; economic impact; Female; Fundamental and applied biological sciences. Psychology; immune response; Immunization; immunoglobulin G; Immunoglobulin G - blood; Immunoglobulin G - immunology; inflammation; Jacalin; jackfruits; Lectins; lethal dose; livestock and meat industry; Mannose-Binding Lectins - immunology; Mannose-Binding Lectins - pharmacology; Mice; Mice, Inbred C57BL; Microbiology; morbidity; mortality; Neospora - pathogenicity; Neospora caninum; neosporosis; neuromuscular disorders; Parasite Load; parasites; pathogens; Plant Lectins - immunology; Plant Lectins - pharmacology; reproductive disorders; Spleen - cytology; Spleen - immunology; splenocytes; vaccines; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Jacalin; Lectins; ArtinM; Immunization; Neospora caninum; Protozoa; Lectin; Apicomplexa; Mannose; Vaccine; Moraceae; Woody plant; Artocarpus; Dicotyledones; Immunological adjuvant; Angiospermae; Spermatophyta
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2011.09.136

Highlights ► We evaluate the adjuvant effect of ArtinM and Jacalin in vaccination for neosporosis. ► Mice were immunized with Neospora antigen (NLA) associated or not with lectins. ► ArtinM increased NLA immunogenicity, IgG2a/IgG1 ratio, Th1 cytokine production. ► After challenge, ArtinM increased survival and decreased brain parasite burden. ► ArtinM showed stronger adjuvant effect than Jacalin in neosporosis vaccination.