DMY gene induces male development in genetically female (XX) medaka fish

Although the sex-determining gene SRY/Sry has been identified in mammals, homologues and genes that have a similar function have yet to be identified in nonmammalian vertebrates. Recently, DMY (the DM-domain gene on the Y chromosome) was cloned from the sex-determining region on the Y chromosome of...

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Published inProceedings of the National Academy of Sciences - PNAS Vol. 104; no. 10; pp. 3865 - 3870
Main Authors Matsuda, Masaru, Shinomiya, Ai, Kinoshita, Masato, Suzuki, Aya, Kobayashi, Tohru, Paul-Prasanth, Bindhu, Lau, En-lieng, Hamaguchi, Satoshi, Sakaizumi, Mitsuru, Nagahama, Yoshitaka
Format Journal Article
LanguageEnglish
Published United States National Academy of Sciences 06.03.2007
National Acad Sciences
Subjects
Animal reproduction
Animals
Animals, Genetically Modified
Base Sequence
Biological Sciences
Chromosomes
Cloning
Cytomegalovirus
Deoxyribonucleic acid
DNA
DNA, Complementary - metabolism
Embryos
Female
Fish
Freshwater
Genes
Genes, sry
Genomics
Germ cells
Gonads
Male
Models, Genetic
Molecular Sequence Data
Oryzias - genetics
Oryzias latipes
Phenotype
Promoter Regions, Genetic
Sex chromosomes
Sex Determination Processes
Sex Differentiation
Somatic cells
sry genes
Teleostei
Transgenic animals
X Chromosome
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Summary:Although the sex-determining gene SRY/Sry has been identified in mammals, homologues and genes that have a similar function have yet to be identified in nonmammalian vertebrates. Recently, DMY (the DM-domain gene on the Y chromosome) was cloned from the sex-determining region on the Y chromosome of the teleost fish medaka (Oryzias latipes). DMY has been shown to be required for the normal development of male individuals. In this study, we show that a 117-kb genomic DNA fragment that carries DMY is able to induce testis differentiation and subsequent male development in XX (genetically female) medaka. In addition, overexpression of DMY cDNA under the control of the CMV promoter also caused XX sex reversal. These results demonstrate that DMY is sufficient for male development in medaka and suggest that the functional difference between the X and Y chromosomes in medaka is a single gene. Our data indicate that DMY is an additional sex-determining gene in vertebrates.
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Author contributions: M.M., A. Shinomiya, A. Suzuki, M.S., and Y.N. designed research; M.M., A. Shinomiya, M.K., A. Suzuki, T.K., B.P.-P., E.-l.L., and M.S. performed research; M.K. contributed new reagents/analytic tools; M.M., A. Shinomiya, A. Suzuki, and T.K. analyzed data; and M.M., A. Shinomiya, A. Suzuki, T.K., B.P.-P., S.H., M.S., and Y.N. wrote the paper.
Communicated by Howard A. Bern, University of California, Berkeley, CA, January 3, 2007
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.0611707104