The SRA protein UHRF1 promotes epigenetic crosstalks and is involved in prostate cancer progression

• Published in: Oncogene Vol. 31; no. 46; pp. 4878 - 4887
• Main Authors: Babbio, F, Pistore, C, Curti, L, Castiglioni, I, Kunderfranco, P, Brino, L, Oudet, P, Seiler, R, Thalman, G N, Roggero, E, Sarti, M, Pinton, S, Mello-Grand, M, Chiorino, G, Catapano, C V, Carbone, G M, Bonapace, I M
• Format: Journal Article
• Language: English
• Published: England Nature Publishing Group 15.11.2012; Nature Publishing Group [1987-....]
• Subjects: Acetylation; Analysis; Biochemistry, Molecular Biology; CCAAT-Enhancer-Binding Proteins - genetics; CCAAT-Enhancer-Binding Proteins - metabolism; Cell Growth Processes - physiology; Cell Line, Tumor; Cell proliferation; Cellular biology; Cellular proteins; Data processing; Development and progression; Disease Progression; DNA Methylation; DNA methyltransferase; DNA microarrays; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; Enhancer of Zeste Homolog 2 Protein; Epigenesis, Genetic; Epigenetic inheritance; Epigenetics; Gene expression; Gene Expression Regulation, Neoplastic; Gene Silencing; Genes; Genes, Tumor Suppressor; Genetic aspects; HEK293 Cells; Histone methyltransferase; Histone Methyltransferases; Histone-Lysine N-Methyltransferase - genetics; Histone-Lysine N-Methyltransferase - metabolism; Histones; Histones - genetics; Histones - metabolism; Humans; Immunohistochemistry; Immunoprecipitation; Immunoprecipitation - methods; Life Sciences; Male; Methylation; Pathogenesis; Physiological aspects; Polycomb Repressive Complex 2 - genetics; Polycomb Repressive Complex 2 - metabolism; Promoter Regions, Genetic; Promoters; Prostate cancer; Prostatectomy; Prostatic Neoplasms - genetics; Prostatic Neoplasms - metabolism; Prostatic Neoplasms - pathology; Proteins; Risk factors; Transformation; Tumor suppressor genes; Tumors; Italy; Switzerland
• ISSN: 0950-9232; 1476-5594
• DOI: 10.1038/onc.2011.641

Epigenetic silencing of tumour suppressor genes is an important mechanism involved in cell transformation and tumour progression. The Set and RING-finger-associated domain-containing protein UHRF1 might be an important link between different epigenetic pathways. Here, we report that UHRF1 is frequently overexpressed in human prostate tumours and has an important role in prostate cancer pathogenesis and progression. Analysis of human prostate cancer samples by microarrays and immunohistochemistry showed increased expression of UHRF1 in about half of the cases. Moreover, UHRF1 expression was associated with reduced overall survival after prostatectomy in patients with organ-confined prostate tumours (P < 0.0001). UHRF1 expression was negatively correlated with several tumour suppressor genes and positively with the histone methyltransferase (HMT) EZH2 both in prostate tumours and cell lines. UHRF1 knockdown reduced proliferation, clonogenic capability and anchorage-independent growth of prostate cancer cells. Depletion of UHRF1 resulted in reactivation of several tumour suppressor genes. Gene reactivation upon UHRF1 depletion was associated with changes in histone H3K9 methylation, acetylation and DNA methylation, and impaired binding of the H3K9 HMT Suv39H1 to the promoter of silenced genes. Co-immunoprecipitation experiments showed direct interaction between UHRF1 and Suv39H1. Our data support the notion that UHRF1, along with Suv39H1 and DNA methyltransferases, contributes to epigenetic gene silencing in prostate tumours. This could represent a parallel and convergent pathway to the H3K27 methylation catalyzed by EZH2 to synergistically promote inactivation of tumour suppressor genes. Deregulated expression of UHRF1 is involved in the prostate cancer pathogenesis and might represent a useful marker to distinguish indolent cancer from those at high risk of lethal progression.