Blocking effects of polyunsaturated fatty acids on Na+ channels of neonatal rat ventricular myocytes

Recent evidence indicates that polyunsaturated long-chain fatty acids (PUFAs) prevent lethal ischemia-induced cardiac arrhythmias in animals and probably in humans. To increase understanding of the mechanism(s) of this phenomenon, the effects of PUFAs on Na+ currents were assessed by the whole-cell...

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Published inProceedings of the National Academy of Sciences - PNAS Vol. 92; no. 24; pp. 11000 - 11004
Main Authors Xiao, Y.F. (Harvard Medical School, Boston, MA.), Kang, J.X, Morgan, J.P, Leaf, A
Format Journal Article
LanguageEnglish
Published United States National Academy of Sciences of the United States of America 21.11.1995
National Acad Sciences
Subjects
ACIDE GRAS INSATURE
ACIDE GRAS POLYINSATURE
ACIDOS GRASOS INSATURADOS
ACIDOS GRASOS POLIINSATURADOS
Animals
Antiarrhythmics
Cells, Cultured
CELLULE
CELULAS
COEUR
CORAZON
Dysrhythmias
Eicosapentaenoic Acid - pharmacology
Electric current
Electric potential
ENFERMEDADES CARDIOVASCULARES
ESTRUCTURA CELULAR
Fatty acids
Fatty Acids, Unsaturated - pharmacology
Heart - physiology
INHIBICION
INHIBITION
ION
Ion Channel Gating - drug effects
IONES
MALADIE CARDIOVASCULAIRE
Membrane Potentials
Myocardium - cytology
Nonesterified fatty acids
Omega 6 fatty acids
Patch-Clamp Techniques
RAT
RATA
Rats
SODIO
SODIUM
Sodium - physiology
Sodium Channels - drug effects
Steady state current
STRUCTURE CELLULAIRE
Time dependence
Time Factors
Unsaturated fatty acids
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Summary:Recent evidence indicates that polyunsaturated long-chain fatty acids (PUFAs) prevent lethal ischemia-induced cardiac arrhythmias in animals and probably in humans. To increase understanding of the mechanism(s) of this phenomenon, the effects of PUFAs on Na+ currents were assessed by the whole-cell patch-clamp technique in cultured neonatal rat ventricular myocytes. Extracellular application of the free 5,8,11,14,17-eicosapentaenoic acid (EPA) produced a concentration-dependent suppression of ventricular, voltage-activated Na+ currents (I(Na)). After cardiac myocytes were treated with 5 or 10 micromolar EPA, the peak I(Na) (elicited by a single-step voltage change with pulses from -80 to -30 mV) was decreased by 51% +/- 8% (P 0.01; n
Bibliography:9600495
S30
ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.92.24.11000