Degradation of n-alkanes and polycyclic aromatic hydrocarbons in petroleum by a newly isolated Pseudomonas aeruginosa DQ8

• Published in: Bioresource technology Vol. 102; no. 5; pp. 4111 - 4116
• Main Authors: Zhang, Zhengzhi, Hou, Zhaowei, Yang, Chunyu, Ma, Cuiqing, Tao, Fei, Xu, Ping
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.03.2011; [New York, NY]: Elsevier Ltd; Elsevier
• Subjects: Alkanes - analysis; Alkanes - metabolism; Bacteria; Biodegradation; Biodegradation, Environmental; Biological and medical sciences; Biotechnology; Crude oil; Degradation; Fluorenes - metabolism; Fundamental and applied biological sciences. Psychology; Gas Chromatography-Mass Spectrometry; Metabolites; n-Alkane; Oxidation-Reduction; Pathways; Petroleum; Petroleum - analysis; Polyallylamine hydrochloride; Polycyclic aromatic hydrocarbons; Polycyclic Aromatic Hydrocarbons - analysis; Polycyclic Aromatic Hydrocarbons - metabolism; Pseudomonas aeruginosa; Pseudomonas aeruginosa - metabolism; Biodegradation; Pseudomonas aeruginosa; Polycyclic aromatic hydrocarbons; Petroleum; n-Alkane; Pseudomonadales; Hydrocarbon; Alkane; Bacteria; Pseudomonadaceae; Polycyclic aromatic compound
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2010.12.064

A bacterial isolate, designated as DQ8, was found capable of degrading diesel, crude oil, n-alkanes and polycyclic aromatic hydrocarbons (PAHs) in petroleum. Strain DQ8 was assigned to the genus Pseudomonas aeruginosa based on biochemical and genetic data. The metabolites identified from n-docosane as substrate suggested that P. aeruginosa DQ8 could oxidize n-alkanes via a terminal oxidation pathway. P. aeruginosa DQ8 could also degrade PAHs of three or four aromatic rings. The metabolites identified from fluorene as substrate suggested that P. aeruginosa DQ8 may degrade fluorene via two pathways. One is monooxygenation at C-9 of fluorene, and the other is initiated by dioxygenation at C-3 and C-4 of fluorene. P. aeruginosa DQ8 should be of great practical significance both in bioremediation of oil-contaminated soils and biotreatment of oil wastewater.