Effect of strontium-substituted biphasic calcium phosphate on inflammatory mediators production by human monocytes

In contact or when engulfed by monocytes wear debris (▪) induce a production of several mediators including pro-inflammatory (TNF-α, IL-1β, IL-6 and IL-18), chemotactic (IL-8) and anti-inflammatory (IL-10) ones. All these factors act in balance with each other and with proteases (MMP-9 ▪) and their...

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Published inActa biomaterialia Vol. 8; no. 8; pp. 3113 - 3119
Main Authors Buache, E., Velard, F., Bauden, E., Guillaume, C., Jallot, E., Nedelec, J.M., Laurent-Maquin, D., Laquerriere, P.
Format Journal Article
LanguageEnglish
Published England Elsevier Ltd 01.08.2012
Elsevier
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Summary:In contact or when engulfed by monocytes wear debris (▪) induce a production of several mediators including pro-inflammatory (TNF-α, IL-1β, IL-6 and IL-18), chemotactic (IL-8) and anti-inflammatory (IL-10) ones. All these factors act in balance with each other and with proteases (MMP-9 ▪) and their inhibitors (TIMP-1 ▪) to ensure tissue homeostasis and renewal (ie. osteoclastogenesis). The use of strontium-substituted BCP (▪) can decrease the production of inflammatory cytokines (TNF-α and IL-6), chemokine (IL-8) and proteases inhibitor (TIMP-1). This allows a fine tuning of inflammatory response which cannot be exacerbated due to maintained IL-10 concentration. Moreover, BCP5% favors matrix turnover by decreased TIMP-1 and increased MMP-9 production. Calcium phosphate materials are widely used as bone substitutes because of their properties close to those of the mineral phase of bones. Nevertheless, after several months, calcium phosphate-based materials release particles that may be phagocytosed by monocytes, leading to an inflammatory reaction. Strontium is well known to counteract the osteoporosis process, but little is known about its effect on inflammatory processes. The purpose of this work was to study the effect of biphasic calcium phosphate (BCP) particles substituted with strontium on the inflammatory reaction. Human primary monocytes stimulated or not by lipopolysaccharide (LPS) were exposed to BCP particles containing strontium for 6 and 24h. Inflammatory mediators (cytokines and matrix metalloproteinases (MMPs)) production was then quantified by ELISA and zymography. We observed that the presence of strontium had few effects on unstimulated cells, but it decreased the production of pro-inflammatory cytokines and the chemokine interleukin 8 in LPS-stimulated cell-conditioned medium. This work suggests for the first time that strontium may be involved in the control of inflammatory processes following BCP phagocytosis by human monocytes.
Bibliography:http://dx.doi.org/10.1016/j.actbio.2012.04.045
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ISSN:1742-7061
1878-7568
DOI:10.1016/j.actbio.2012.04.045