Identification of the Marek's disease virus serotype 2 genes homologous to the glycoprotein B (UL27), ICP18.5 (UL28) and major DNA-binding protein (UL29) genes of herpes simplex virus type 1

• Published in: Journal of Veterinary Medical Science Vol. 61; no. 10; pp. 1161 - 1165
• Main Authors: Kato, K. (Tokyo Univ. (Japan). Faculty of Agriculture), Jang, H.K, Izumiya, Y, Cai, J.S, Tsushima, Y, Miyazawa, T, Kai, C, Mikami, T
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.10.1999; Japan Science and Technology Agency
• Subjects: ADN; Amino Acid Sequence; Animals; BINDING PROTEINS; Blotting, Northern; CHICKENS; Consensus Sequence; DNA; DNA Probes - chemistry; Electrophoresis, Agar Gel; GENE; Gene Library; GENES; GLICOPROTEINAS; GLYCOPROTEINE; GLYCOPROTEINS; Herpes simplex virus 1; Herpesvirus 1, Human - chemistry; Herpesvirus 1, Human - genetics; Herpesvirus 2, Gallid - chemistry; Herpesvirus 2, Gallid - genetics; HERPETOVIRIDAE; Marek Disease - genetics; Marek's disease herpesvirus; MAREK'S DISEASE VIRUS; Marek’s disease virus serotype 2; Molecular Sequence Data; POLLO; POULET; PROTEINAS AGLUTINANTES; PROTEINE DE LIAISON; RNA, Viral - isolation & purification; Sequence Alignment; Sequence Analysis, DNA; Sequence Homology, Amino Acid; UL27-UL29; VIRUS ENFERMEDAD DE MAREK; VIRUS MALADIE DE MAREK
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.61.1161

We determined the nucleotide sequence of non-pathogenic Marek's disease virus serotype 2 (MDV2) strain HPRS24 glycoprotein B (gB) (UL27), ICP18.5 (UL28) and major DNA-binding protein (MDBP) (UL29) genes homologous to herpes simplex virus type 1 (HSV-1). The sequence data revealed that important motives in the proteins are conserved in MDV2 ICP18.5 and MDBP, however the sequence of viral DNA replication origin which exists in the regions between the UL29 and UL30 genes of other alphaherpesviruses was not found in the regions of the MDV2 genome. By northern blot analyses, we also demonstrated that 8.9, 5.0 and 2.6 kb transcripts were actually transcribed from the sequenced region in MDV2-infected cells. The MDV2 UL28 and UL29 genes have not been reported in other serotypes of MDV