Neutrophil secretion products regulate anti‐bacterial activity in monocytes and macrophages

• Published in: Clinical and experimental immunology Vol. 151; no. 1; pp. 139 - 145
• Main Authors: Soehnlein, O., Kenne, E., Rotzius, P., Eriksson, E. E., Lindbom, L.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.01.2008; Blackwell; Blackwell Science Inc
• Subjects: Analysis of Variance; Analytical, structural and metabolic biochemistry; anti‐microbial activity; Bacterial Infections - immunology; Basic Immunology; Biological and medical sciences; Blood Bactericidal Activity; Cells, Cultured; Fundamental and applied biological sciences. Psychology; granule protein; Humans; innate immunity; macrophage; Macrophage Activation; Macrophages - physiology; Medicin och hälsovetenskap; Microscopy, Fluorescence; Monocytes - physiology; neutrophil; Neutrophils - metabolism; Phagocytosis; Reactive Oxygen Species - analysis; Respiratory Burst; Monocyte; Secretion; Biochemistry; Immunity; Protein; Infection; Regulation(control); innate immunity; Bacteriosis; granule protein; Neutrophil; anti-microbial activity; Macrophage
• ISSN: 0009-9104; 1365-2249; 1365-2249
• DOI: 10.1111/j.1365-2249.2007.03532.x

Summary Macrophages represent a multi‐functional cell type in innate immunity that contributes to bacterial clearance by recognition, phagocytosis and killing. In acute inflammation, infiltrating neutrophils release a wide array of preformed granule proteins which interfere functionally with their environment. Here, we present a novel role for neutrophil‐derived granule proteins in the anti‐microbial activity of macrophages. Neutrophil secretion obtained by antibody cross‐linking of the integrin subunit CD18 (X‐link secretion) or by treatment with N‐Formyl‐Met‐Leu‐Phe (fMLP secretion) induced a several‐fold increase in bacterial phagocytosis by monocytes and macrophages. This response was associated with a rapid activation of the monocytes and macrophages as depicted by an increase in cytosolic free Ca2+. Interestingly, fMLP secretion had a more pronounced effect on monocytes than the X‐link secretion, while the opposite was observed for macrophages. In addition, polymorphonuclear cells (PMN) secretion caused a strong enhancement of intracellular reactive oxygen species (ROS) formation compared to incubation with bacteria. Thus, secretion of neutrophil granule proteins activates macrophages to increase the phagocytosis of bacteria and to enhance intracellular ROS formation, indicating pronounced intracellular bacterial killing. Both mechanisms attribute novel microbicidal properties to PMN granule proteins, suggesting their potential use in anti‐microbial therapy.