Microarray analysis of acaricide-inducible gene expression in the southern cattle tick, Rhipicephalus (Boophilus) microplus

• Published in: Insect molecular biology Vol. 17; no. 6; pp. 597 - 606
• Main Authors: Saldivar, L, Guerrero, F. D, Miller, R. J, Bendele, K. G, Gondro, C, Brayton, K. A
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.12.2008
• Subjects: acaricide resistance; amitraz; Animals; Boophilus microplus; coumaphos; Coumaphos - toxicity; detoxification enzymes; DNA Primers - genetics; gene expression; gene expression regulation; Gene Expression Regulation - drug effects; genes; glutathione transferase; ivermectin; Ivermectin - toxicity; Ixodidae; larvae; legumain; microarray technology; microarrays; Oligonucleotide Array Sequence Analysis; organophosphates; permethrin; Permethrin - toxicity; Pesticides - toxicity; proteins; Reverse Transcriptase Polymerase Chain Reaction; Rhipicephalus; Rhipicephalus (Boophilus) microplus; Ticks - genetics; Ticks - metabolism; Toluidines - toxicity
• ISSN: 1365-2583; 0962-1075; 1365-2583
• DOI: 10.1111/j.1365-2583.2008.00831.x

Acaricide-inducible differential gene expression was studied in larvae of Rhipicephalus (Boophilus) microplus using a microarray-based approach. The acaricides used were: coumaphos, permethrin, ivermectin, and amitraz. The microarrays contained over 13 000 probes, having been derived from a previously described R. microplus gene index (BmiGI Version 2; Wang et al., 2007). Relative quantitative reverse transcriptase-PCR, real time PCR, and serial analysis of gene expression data was used to verify microarray data. Among the differentially expressed genes with informative annotation were legumain, glutathione S-transferase, and a putative salivary gland-associated protein.