Characterization of tomato zonate spot virus, a new tospovirus in China

• Published in: Archives of virology Vol. 153; no. 5; pp. 855 - 864
• Main Authors: Dong, Jia-Hong, Cheng, Xiao-Fei, Yin, Yue-Yan, Fang, Qi, Ding, Ming, Li, Ting-Ting, Zhang, Li-Zhen, Su, Xiao-Xia, McBeath, Jenifer Huang, Zhang, Zhong-Kai
• Format: Journal Article
• Language: English
• Published: Vienna Vienna : Springer Vienna 01.05.2008; Springer Vienna; Springer; Springer Nature B.V
• Subjects: Amino Acid Motifs; Amino Acid Sequence; Base Sequence; Biological and medical sciences; Biomedical and Life Sciences; Biomedicine; Biotechnology; Calla; Capsicum - virology; China; Cloning, Molecular; Conserved Sequence; Crop diseases; DNA Primers - genetics; Fruits; Fundamental and applied biological sciences. Psychology; Genomes; Infectious Diseases; Lycopersicon esculentum; Lycopersicon esculentum - virology; Medical Microbiology; Microbiology; Microscopy; Microscopy, Electron, Transmission; Miscellaneous; Molecular Sequence Data; Original Article; Phylogenetics; Phylogeny; Phytopathology. Animal pests. Plant and forest protection; Plant Diseases - virology; Plant viruses and viroids; Protein Structure, Tertiary; Proteins; Reverse Transcriptase Polymerase Chain Reaction; RNA polymerase; RNA, Viral - chemistry; RNA, Viral - genetics; Sequence Homology, Amino Acid; Serology; Terminology as Topic; Tomatoes; Tospovirus; Tospovirus - classification; Tospovirus - genetics; Tospovirus - isolation & purification; Tospovirus - ultrastructure; Viral Proteins - chemistry; Viral Proteins - genetics; Virology; Viruses; Asia; China; Tomato Spot Wilt Virus; Iris Yellow Spot Virus; Impatiens Necrotic Spot Virus; Melon Yellow Spot Virus; Virus; Bunyaviridae; Plant pathogen; Vegetable crop; Dicotyledones; Tospovirus; Angiospermae; Lycopersicon esculentum; Spermatophyta; Solanaceae
• ISSN: 0304-8608; 1432-8798
• DOI: 10.1007/s00705-008-0054-5

An isolate of a new tospovirus species, causing concentric zoned ringspots on fruits and necrotic lesions on leaves of infected plants, was characterised based on particle morphology, host range and serological properties. The complete nucleotide sequences of large (L), medium (M), and small (S) RNAs of this virus were found to contain 8919, 4945, and 3279 nts respectively. The L RNA encoded the RNA-dependent RNA polymerase (RdRp) (2885 aa, 332.7 kDa). The M RNA encoded a non-structural (NSm) protein (309 aa, 34.4 kDa) and a viral glycoprotein precursor (Gn/Gc) (1122 aa, 127.4 kDa). The S RNA encoded a non-structural protein (NSs) (459 aa, 51.9 kDa) and the nucleocapsid (N) protein (278 aa, 30.6 kDa). This N protein shared amino acid identities of 80.9% with those of calla lily chlorotic spot virus. Our results suggest that the virus studied here belongs to a new tospovirus species, for which the name tomato zonate spot virus is proposed.