A simple method for the simultaneous decoloration and deproteinization of crude levan extract from Paenibacillus polymyxa EJS-3 by macroporous resin

A simple method for the simultaneous decoloration and deproteinization of crude levan extract from the endophytic bacterium Paenibacillus polymyxa EJS-3 was developed through static and dynamic adsorption tests of macroporous resins. S-8 resin demonstrated the highest decoloration and deproteinizati...

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Published inBioresource technology Vol. 101; no. 15; pp. 6077 - 6083
Main Authors Liu, Jun, Luo, Jianguang, Sun, Yi, Ye, Hong, Lu, Zhaoxin, Zeng, Xiaoxiong
Format Journal Article
LanguageEnglish
Published Kidlington Elsevier Ltd 01.08.2010
[New York, NY]: Elsevier Ltd
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Abstract A simple method for the simultaneous decoloration and deproteinization of crude levan extract from the endophytic bacterium Paenibacillus polymyxa EJS-3 was developed through static and dynamic adsorption tests of macroporous resins. S-8 resin demonstrated the highest decoloration and deproteinization ratios among various resins tested. Under optimized static adsorption conditions (pH 6.0, 35°C and adsorption time of 70min), the ratios of decoloration, deproteinization and polysaccharide recovery for S-8 resin were 76.8%, 78.9% and 69.0%, respectively. Under optimized dynamic adsorption condition (flow rate of 2BV/h, 160ml of 2.5mg/ml crude levan extract), higher ratios of decoloration, deproteinization and polysaccharide recovery for S-8 resin (84.6%, 91.7% and 81.3%, respectively) were observed. The method developed will provide a potential approach for large-scale production of levan from P.polymyxa EJS-3.
AbstractList A simple method for the simultaneous decoloration and deproteinization of crude levan extract from the endophytic bacterium Paenibacillus polymyxa EJS-3 was developed through static and dynamic adsorption tests of macroporous resins. S-8 resin demonstrated the highest decoloration and deproteinization ratios among various resins tested. Under optimized static adsorption conditions (pH 6.0, 35°C and adsorption time of 70min), the ratios of decoloration, deproteinization and polysaccharide recovery for S-8 resin were 76.8%, 78.9% and 69.0%, respectively. Under optimized dynamic adsorption condition (flow rate of 2BV/h, 160ml of 2.5mg/ml crude levan extract), higher ratios of decoloration, deproteinization and polysaccharide recovery for S-8 resin (84.6%, 91.7% and 81.3%, respectively) were observed. The method developed will provide a potential approach for large-scale production of levan from P. polymyxa EJS-3.
A simple method for the simultaneous decoloration and deproteinization of crude levan extract from the endophytic bacterium Paenibacillus polymyxa EJS-3 was developed through static and dynamic adsorption tests of macroporous resins. S-8 resin demonstrated the highest decoloration and deproteinization ratios among various resins tested. Under optimized static adsorption conditions (pH 6.0, 35 degrees C and adsorption time of 70 min), the ratios of decoloration, deproteinization and polysaccharide recovery for S-8 resin were 76.8%, 78.9% and 69.0%, respectively. Under optimized dynamic adsorption condition (flow rate of 2 BV/h, 160 ml of 2.5mg/ml crude levan extract), higher ratios of decoloration, deproteinization and polysaccharide recovery for S-8 resin (84.6%, 91.7% and 81.3%, respectively) were observed. The method developed will provide a potential approach for large-scale production of levan from P.polymyxa EJS-3.A simple method for the simultaneous decoloration and deproteinization of crude levan extract from the endophytic bacterium Paenibacillus polymyxa EJS-3 was developed through static and dynamic adsorption tests of macroporous resins. S-8 resin demonstrated the highest decoloration and deproteinization ratios among various resins tested. Under optimized static adsorption conditions (pH 6.0, 35 degrees C and adsorption time of 70 min), the ratios of decoloration, deproteinization and polysaccharide recovery for S-8 resin were 76.8%, 78.9% and 69.0%, respectively. Under optimized dynamic adsorption condition (flow rate of 2 BV/h, 160 ml of 2.5mg/ml crude levan extract), higher ratios of decoloration, deproteinization and polysaccharide recovery for S-8 resin (84.6%, 91.7% and 81.3%, respectively) were observed. The method developed will provide a potential approach for large-scale production of levan from P.polymyxa EJS-3.
A simple method for the simultaneous decoloration and deproteinization of crude levan extract from the endophytic bacterium Paenibacillus polymyxa EJS-3 was developed through static and dynamic adsorption tests of macroporous resins. S-8 resin demonstrated the highest decoloration and deproteinization ratios among various resins tested. Under optimized static adsorption conditions (pH 6.0, 35 C and adsorption time of 70 min), the ratios of decoloration, deproteinization and polysaccharide recovery for S-8 resin were 76.8%, 78.9% and 69.0%, respectively. Under optimized dynamic adsorption condition (flow rate of 2 BV/h, 160 ml of 2.5 mg/ml crude levan extract), higher ratios of decoloration, deproteinization and polysaccharide recovery for S-8 resin (84.6%, 91.7% and 81.3%, respectively) were observed. The method developed will provide a potential approach for large-scale production of levan from P. polymyxa EJS-3.
A simple method for the simultaneous decoloration and deproteinization of crude levan extract from the endophytic bacterium Paenibacillus polymyxa EJS-3 was developed through static and dynamic adsorption tests of macroporous resins. S-8 resin demonstrated the highest decoloration and deproteinization ratios among various resins tested. Under optimized static adsorption conditions (pH 6.0, 35 degrees C and adsorption time of 70 min), the ratios of decoloration, deproteinization and polysaccharide recovery for S-8 resin were 76.8%, 78.9% and 69.0%, respectively. Under optimized dynamic adsorption condition (flow rate of 2 BV/h, 160 ml of 2.5mg/ml crude levan extract), higher ratios of decoloration, deproteinization and polysaccharide recovery for S-8 resin (84.6%, 91.7% and 81.3%, respectively) were observed. The method developed will provide a potential approach for large-scale production of levan from P.polymyxa EJS-3.
Author Ye, Hong
Luo, Jianguang
Zeng, Xiaoxiong
Liu, Jun
Lu, Zhaoxin
Sun, Yi
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Issue 15
Keywords Adsorption
Levan
Deproteinization
Decoloration
Macroporous resin
Paenibacillus polymyxa
Bacteria
Decolorizing process
Resins
Macroporosity
Language English
License CC BY 4.0
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Snippet A simple method for the simultaneous decoloration and deproteinization of crude levan extract from the endophytic bacterium Paenibacillus polymyxa EJS-3 was...
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SubjectTerms Adsorption
bacteria
Biological and medical sciences
chemistry
Color
Composite Resins
Composite Resins - chemistry
Decoloration
decolorization
Deproteinization
dynamic testing
Fructans
Fructans - chemistry
Fructans - isolation & purification
Fundamental and applied biological sciences. Psychology
isolation & purification
Levan
Macroporous resin
metabolism
methods
Paenibacillus
Paenibacillus - metabolism
Paenibacillus polymyxa
Plant Proteins
Plant Proteins - isolation & purification
Porosity
resins
Ultrafiltration
Ultrafiltration - methods
Title A simple method for the simultaneous decoloration and deproteinization of crude levan extract from Paenibacillus polymyxa EJS-3 by macroporous resin
URI https://dx.doi.org/10.1016/j.biortech.2010.03.019
https://www.ncbi.nlm.nih.gov/pubmed/20346649
https://www.proquest.com/docview/734024749
https://www.proquest.com/docview/742686471
https://www.proquest.com/docview/745931208
Volume 101
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