A simple method for the simultaneous decoloration and deproteinization of crude levan extract from Paenibacillus polymyxa EJS-3 by macroporous resin

• Published in: Bioresource technology Vol. 101; no. 15; pp. 6077 - 6083
• Main Authors: Liu, Jun, Luo, Jianguang, Sun, Yi, Ye, Hong, Lu, Zhaoxin, Zeng, Xiaoxiong
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.08.2010; [New York, NY]: Elsevier Ltd; Elsevier
• Subjects: Adsorption; bacteria; Biological and medical sciences; chemistry; Color; Composite Resins; Composite Resins - chemistry; Decoloration; decolorization; Deproteinization; dynamic testing; Fructans; Fructans - chemistry; Fructans - isolation & purification; Fundamental and applied biological sciences. Psychology; isolation & purification; Levan; Macroporous resin; metabolism; methods; Paenibacillus; Paenibacillus - metabolism; Paenibacillus polymyxa; Plant Proteins; Plant Proteins - isolation & purification; Porosity; resins; Ultrafiltration; Ultrafiltration - methods; Adsorption; Levan; Deproteinization; Decoloration; Macroporous resin; Paenibacillus polymyxa; Bacteria; Decolorizing process; Resins; Macroporosity
• ISSN: 0960-8524; 1873-2976; 1873-2976
• DOI: 10.1016/j.biortech.2010.03.019

A simple method for the simultaneous decoloration and deproteinization of crude levan extract from the endophytic bacterium Paenibacillus polymyxa EJS-3 was developed through static and dynamic adsorption tests of macroporous resins. S-8 resin demonstrated the highest decoloration and deproteinization ratios among various resins tested. Under optimized static adsorption conditions (pH 6.0, 35°C and adsorption time of 70min), the ratios of decoloration, deproteinization and polysaccharide recovery for S-8 resin were 76.8%, 78.9% and 69.0%, respectively. Under optimized dynamic adsorption condition (flow rate of 2BV/h, 160ml of 2.5mg/ml crude levan extract), higher ratios of decoloration, deproteinization and polysaccharide recovery for S-8 resin (84.6%, 91.7% and 81.3%, respectively) were observed. The method developed will provide a potential approach for large-scale production of levan from P.polymyxa EJS-3.