The final assembly of trehalose polyphleates takes place within the outer layer of the mycobacterial cell envelope

• Published in: The Journal of biological chemistry Vol. 295; no. 32; pp. 11184 - 11194
• Main Authors: Thouvenel, Laurie, Prevot, Gautier, Chiaradia, Laura, Parra, Julien, Mouton-Barbosa, Emmanuelle, Locard-Paulet, Marie, Marcoux, Julien, Tropis, Maryelle, Burlet-Schiltz, Odile, Daffé, Mamadou, Guilhot, Christophe, Etienne, Gilles, Chalut, Christian
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 07.08.2020; American Society for Biochemistry and Molecular Biology
• Subjects: acyltransferase; Biochemistry, Molecular Biology; Cell Membrane - metabolism; enzyme; glycolipid; Glycolipids - metabolism; Life Sciences; lipid synthesis; lipid transport; membrane biogenesis; Microbiology; Microbiology and Parasitology; Mycobacterium smegmatis; Mycobacterium smegmatis - metabolism; Mycobacterium tuberculosis - metabolism; protein export; Proteolysis; proteomics; subcellular fractionation; Subcellular Fractions - metabolism; Trehalose - metabolism; trehalose polyphleate; enzyme; glycolipid; lipid synthesis; protein export; proteomics; subcellular fractionation; lipid transport; Mycobacterium smegmatis; membrane biogenesis; acyltransferase; trehalose polyphleate
• ISSN: 0021-9258; 1083-351X; 1083-351X
• DOI: 10.1074/jbc.RA120.013299

Trehalose polyphleates (TPP) are high-molecular-weight, surface-exposed glycolipids present in a broad range of nontuberculous mycobacteria. These compounds consist of a trehalose core bearing polyunsaturated fatty acyl substituents (called phleic acids) and a straight-chain fatty acid residue and share a common basic structure with trehalose-based glycolipids produced by Mycobacterium tuberculosis. TPP production starts in the cytosol with the formation of a diacyltrehalose intermediate. An acyltransferase, called PE, subsequently catalyzes the transfer of phleic acids onto diacyltrehalose to form TPP, and an MmpL transporter promotes the export of TPP or its precursor across the plasma membrane. PE is predicted to be an anchored membrane protein, but its topological organization is unknown, raising questions about the subcellular localization of the final stage of TPP biosynthesis and the chemical nature of the substrates that are translocated by the MmpL transporter. Here, using genetic, biochemical, and proteomic approaches, we established that PE of Mycobacterium smegmatis is exported to the cell envelope following cleavage of its signal peptide and that this process is required for TPP biosynthesis, indicating that the last step of TPP formation occurs in the outer layers of the mycobacterial cell envelope. These results provide detailed insights into the molecular mechanisms controlling TPP formation and transport to the cell surface, enabling us to propose an updated model of the TPP biosynthetic pathway. Because the molecular mechanisms of glycolipid production are conserved among mycobacteria, these findings obtained with PE from M. smegmatis may offer clues to glycolipid formation in M. tuberculosis.