Processing Pathway Deduced from the Structures of N-Glycans in Carica papaya
A processing The processing pathway of N-glycans in Carica papaya was deduced from the structures of N-glycans. The N-glycans were liberated by hydrazinolysis followed by N-acetylation. Their reducing-end sugar residues were tagged with 2-aminopyridine and the pyridylamino (PA-) sugar chains thus ob...
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Published in | Journal of biochemistry (Tokyo) Vol. 127; no. 6; pp. 1121 - 1126 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
England
Oxford University Press
01.06.2000
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Subjects | |
Online Access | Get full text |
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Summary: | A processing The processing pathway of N-glycans in Carica papaya was deduced from the structures of N-glycans. The N-glycans were liberated by hydrazinolysis followed by N-acetylation. Their reducing-end sugar residues were tagged with 2-aminopyridine and the pyridylamino (PA-) sugar chains thus obtained were purified by HPLC. Eleven PA-sugar chains were found, and their structures were analyzed by two-dimensional sugar mapping combined with partial acid hydrolysis and exoglycosidase digestion. The structures of the N-glycans were of the highmsnnose types with xylose and fucose; however, among them two new N-glycans, Manα1-6(Manα1-3)Manα1-6(Xylβ1-2)Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc and Manα1-3Manα1-6(Xylβ1-2)Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc, were found. Judging from these structures together with Manα1-6(Manα1-3)Manα1-6(Manα1-3)(Xylβ1-2)Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc reported previously [Shimazaki, A., Makino, Y., Omichi, K., Odani, S., and Hase, S. (1999) J. Biochem. 125, 560–565], a processing pathway for N-glycans in C. papaya is inferred in which the activity of Golgi α-mannosidase II is incomplete. |
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Bibliography: | 1This work was supported in part by grants from the Research for the Future Program of the Japan Society for the Promotion of Science, and a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan. istex:21C9FBB45F6F69AA4C6319944367BCE1A96A057C ark:/67375/HXZ-8665KSV7-W ArticleID:127.6.1121 2To whom correspondence should be addressed ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0021-924X 1756-2651 |
DOI: | 10.1093/oxfordjournals.jbchem.a022707 |