Native plants ( Phellodendron amurense and Humulus japonicus ) extracts act as antioxidants to support developmental competence of bovine blastocysts

• Published in: Animal bioscience Vol. 30; no. 9; pp. 1245 - 1252
• Main Authors: Do, Geon-Yeop, Kim, Jin-Woo, Park, Hyo-Jin, Yoon, Seung-Bin, Park, Jae-Young, Yang, Seul-Gi, Jung, Bae Dong, Kwon, Yong-Soo, Kang, Man-Jong, Song, Bong-Seok, Kim, Sun-Uk, Chang, Kyu-Tae, Koo, Deog-Bon
• Format: Journal Article
• Language: English
• Published: Korea (South) Asian - Australasian Association of Animal Production Societies 01.09.2017; Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST); Asian-Australasian Association of Animal Production Societies; 아세아·태평양축산학회
• Subjects: Antioxidants; Antioxidants (Nutrients); Blastocyst; Bovine Embryo; Embryonic development; Hops; Observations; Physiological aspects; Plant extracts; Reactive Oxygen Species (ROS); Rutaceae; 축산학; Antioxidants; Reactive Oxygen Species (ROS); Bovine Embryo; Phellodendron amurense; Humulus japonicas
• ISSN: 1011-2367; 2765-0189; 1976-5517; 2765-0235
• DOI: 10.5713/ajas.16.0985

( ) and ( ) are closely involved in anti-oxidative response and increasing antioxidant enzymes activities. However, the effects of their extracts on development of preimplantation bovine embryos have not been investigated. Therefore, we investigated the effects of and extracts on developmental competence and quality of preimplantation bovine embryos. After fertilization, bovine embryos were cultured for 7 days in Charles Rosenkrans amino acid medium supplemented with (0.01 μg/mL) and (0.01 μg/mL). The effect of this supplementation during culture on development competence and antioxidant was investigated. We observed that the blastocysts rate was significantly increased (p<0.05) in (28.9%±2.9%), (30.9%±1.5%), and a mixture of and (34.8%± 2.1%) treated groups compared with the control group (25.4%±1.6%). We next confirmed that the intracellular levels of reactive oxygen species (ROS) were significantly decreased (p<0.01) in and/or extract treated groups when compared with the control group. Our results also showed that expression of cleaved caspase-3 and apoptotic cells of blastocysts were significantly decreased (p<0.05) in bovine blastocysts derived from both and extract treated embryos. These results suggest that proper treatment with and extracts in the development of preimplantation bovine embryos improves the quality of blastocysts, which may be related to the reduction of ROS level and apoptosis.