Integrated Analysis of Widely Targeted Metabolomics and Transcriptomics Reveals the Effects of Transcription Factor NOR-like1 on Alkaloids, Phenolic Acids, and Flavonoids in Tomato at Different Ripening Stages

Tomato is abundant in alkaloids, phenolic acids, and flavonoids; however, the effect of transcription factor NOR-like1 on these metabolites in tomato is unclear. We used a combination of widely targeted metabolomics and transcriptomics to analyze wild-type tomatoes and CR-NOR-like1 tomatoes. A total...

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Published inMetabolites Vol. 12; no. 12; p. 1296
Main Authors Yang, Xinyu, Zhao, Xiaodan, Fu, Daqi, Zhao, Ying
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 01.12.2022
MDPI
Subjects
Acids
Alkaloids
Bioflavonoids
Biosynthesis
Botanical research
Chemical properties
Correlation analysis
Crops
Discriminant analysis
Flavones
Flavonoids
Fruits
Genetic aspects
Metabolism
Metabolites
Metabolomics
NOR-like1
Phenolic acids
Physiological aspects
Plant genetics
Ripening
Shelf life
tomato
Tomatoes
Transcription factors
Transcriptomics
widely targeted metabolomics
China
United States > US
Germany
widely targeted metabolomics
alkaloids
phenolic acids
transcriptomics
tomato
NOR-like1
flavonoids
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Summary:Tomato is abundant in alkaloids, phenolic acids, and flavonoids; however, the effect of transcription factor NOR-like1 on these metabolites in tomato is unclear. We used a combination of widely targeted metabolomics and transcriptomics to analyze wild-type tomatoes and CR-NOR-like1 tomatoes. A total of 83 alkaloids, 85 phenolic acids, and 96 flavonoids were detected with significant changes. Combined with a KEGG enrichment analysis, we revealed 16 differentially expressed genes (DEGs) in alkaloid-related arginine and proline metabolism, 60 DEGs were identified in the phenolic acid-related phenylpropane biosynthesis, and 30 DEGs were identified in the flavonoid-related biosynthesis pathway. In addition, some highly correlated differential-expression genes with differential metabolites were further identified by correlation analysis. The present research provides a preliminary view of the effects of NOR-like1 transcription factor on alkaloid, phenolic acid, and flavonoid accumulation in tomatoes at different ripening stages based on widely targeted metabolomics and transcriptomics in plants, laying the foundation for extending fruit longevity and shelf life as well as cultivating stress-resistant plants.
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ISSN:2218-1989
2218-1989
DOI:10.3390/metabo12121296