Activity of plasma membrane H(+)-ATPase and optimal glycolytic flux are required for rapid adaptation and growth of Saccharomyces cerevisiae in the presence of the weak-acid preservative sorbic acid

• Published in: Applied and Environmental Microbiology Vol. 62; no. 9; pp. 3158 - 3164
• Main Authors: Holyoak, C.D. (Colworth Laboratory, Shambrook, Bedford, UK.), Stratford, M, McMullin, Z, Cole, M.B, Crimmins, K, Brown, A.J.P, Coote, P.J
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.09.1996
• Subjects: ACIDE SORBIQUE; ACIDEZ; ACIDITE; ACIDO SORBICO; ACTIVIDAD ENZIMATICA; ACTIVITE ENZYMATIQUE; ADAPTACION; ADAPTATION; Adaptation, Physiological; ADENOSINE TRIPHOSPHATE; Adenosine Triphosphate - metabolism; ADENOSINTRIFOSFATO; Biological and medical sciences; Biology of microorganisms of confirmed or potential industrial interest; Biotechnology; Carbon Dioxide - metabolism; CATION; CATIONES; ESTRES; ESTRUCTURA CELULAR; Food Preservatives - pharmacology; Fundamental and applied biological sciences. Psychology; GLICOLISIS; GLYCOLYSE; Glycolysis; HIDROGENO; HIDROLASAS; Hydrogen-Ion Concentration; HYDROGENE; HYDROLASE; ION; IONES; Mission oriented research; MUTANT; MUTANTES; Oxygen Consumption; Physiology and metabolism; PIRUVATO QUINASA; Proton-Translocating ATPases - metabolism; PYRUVATE KINASE; SACCHAROMYCES CEREVISIAE; Saccharomyces cerevisiae - drug effects; Saccharomyces cerevisiae - physiology; Sorbic Acid - pharmacology; STRESS; STRUCTURE CELLULAIRE; TRANSFERASAS; TRANSFERASE; Culture medium; 2,4-Hexadienoic acid; Microorganism growth; Yeast; Enzyme; H; transporting ATP synthase; Mechanism; Fungi; Enzymatic activity; Plasma membrane; Microorganism culture; pH; Hydrolases; Ascomycetes; Inhibition; Saccharomyces cerevisiae; Thallophyta; Adaptation
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/aem.62.9.3158-3164.1996

The weak acid sorbic acid transiently inhibited the growth of Saccharomyces cerevisiae in media at low pH. During a lag period, the length of which depended on the severity of this weak-acid stress, yeast cells appeared to adapt to this stress, eventually recovering and growing normally. This adaptation to weak-acid stress was not due to metabolism and removal of the sorbic acid. A pma1-205 mutant, with about half the normal membrane H+-ATPase activity, was shown to be more sensitive to sorbic acid than its parent. Sorbic acid appeared to stimulate plasma membrane H+-ATPase activity in both PMA1 and pma1-205. Consistent with this, cellular ATP levels showed drastic reductions, the extent of which depended on the severity of weak-acid stress. The weak acid did not appear to affect the synthesis of ATP because CO2 production and O2 consumption were not affected significantly in PMA1 and pma1-205 cells. However, a glycolytic mutant, with about one-third the normal pyruvate kinase and phosphofructokinase activity and hence a reduced capacity to generate ATP, was more sensitive to sorbic acid than its isogenic parent. These data are consistent with the idea that adaptation by yeast cells to sorbic acid is dependent on (i) the restoration of internal pH via the export of protons by the membrane H+-ATPase in an energy-demanding process and (ii) the generation of sufficient ATP to drive this process and still allow growth