New Aspects of the Antioxidant Activity of Glycyrrhizin Revealed by the CIDNP Technique

• Published in: Antioxidants Vol. 11; no. 8; p. 1591
• Main Authors: Ageeva, Aleksandra A, Kruppa, Alexander I, Magin, Ilya M, Babenko, Simon V, Leshina, Tatyana V, Polyakov, Nikolay E
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.08.2022; MDPI
• Subjects: antioxidant activity; Antioxidants; Biological activity; Cancer; Drug delivery systems; Electron transfer; Free radicals; Glycyrrhizin; Intermediates; Naproxen; Nonsteroidal anti-inflammatory drugs; Oxygen; photo-CIDNP; Photodegradation; Severe acute respiratory syndrome coronavirus 2; solvated electron; Ultraviolet radiation; Germany; United States > US; naproxen; 5-sulfosalicylic acid; free radicals; solvated electron; antioxidant activity; photo-CIDNP; glycyrrhizin
• ISSN: 2076-3921; 2076-3921
• DOI: 10.3390/antiox11081591

Electron transfer plays a crucial role in ROS generation in living systems. Molecular oxygen acts as the terminal electron acceptor in the respiratory chains of aerobic organisms. Two main mechanisms of antioxidant defense by exogenous antioxidants are usually considered. The first is the inhibition of ROS generation, and the second is the trapping of free radicals. In the present study, we have elucidated both these mechanisms of antioxidant activity of glycyrrhizin (GL), the main active component of licorice root, using the chemically induced dynamic nuclear polarization (CIDNP) technique. First, it was shown that GL is capable of capturing a solvated electron, thereby preventing its capture by molecular oxygen. Second, we studied the effect of glycyrrhizin on the behavior of free radicals generated by UV irradiation of xenobiotic, NSAID-naproxen in solution. The structure of the glycyrrhizin paramagnetic intermediates formed after the capture of a solvated electron was established from a photo-CIDNP study of the model system-the dianion of 5-sulfosalicylic acid and DFT calculations.