Establishment and Characterization of Feline Mammary Tumor Patient-Derived Xenograft Model

Feline mammary tumor is a relatively commonly diagnosed neoplasm in the cat. Development of new veterinary cancer therapies is limited by the shortage of in vivo models that reproduce tumor microenvironment and metastatic progression. Four feline mammary tumor orthotopic patient-derived xenograft mo...

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Published inAnimals (Basel) Vol. 11; no. 8; p. 2380
Main Authors Chuang, Hsiao-Li, Chang, Yi-Chih, Huang, Yi-Ting, Liao, Jiunn-Wang, Kao, Pei-Ling, Chen, Yi-Fei, Lin, Bin-Yin, Lin, Yi-Lo, Chen, Ter-Hsin, Wang, Yu-Chih
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 12.08.2021
MDPI
Subjects
Cancer therapies
cats
cell line
cell lines
Chemotherapy
feline mammary tumor
immunohistochemistry
Laboratories
liver
lungs
mammary neoplasms (animal)
Medical research
Metastasis
microsatellite repeats
patient-derived xenograft
patients
Tumors
xenotransplantation
United States > US
Taiwan
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Summary:Feline mammary tumor is a relatively commonly diagnosed neoplasm in the cat. Development of new veterinary cancer therapies is limited by the shortage of in vivo models that reproduce tumor microenvironment and metastatic progression. Four feline mammary tumor orthotopic patient-derived xenograft model (PDX) successfully established in NOD-SCID gamma (NSG) mice. The overall success rate of PDX establishment was 36% (4/11). Histological, immunohistochemical, and short tandem repeat analysis showed a remarkable similarity between patient’s tumor and xenograft. The tumor grafts conserve original tumor essential features, including distant metastasis. Primary FMT-1807 cell line isolated from FMT-1807PDX tumor tissue. Tumorigenicity of FMT-1807 cells expanded from PDX was assessed by orthotopic injection into NSG mice. Mice yielded tumors which preserve the lung and liver metastasis ability. This work provides a platform for FMT translational investigation.
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These authors contributed equally to the work.
ISSN:2076-2615
2076-2615
DOI:10.3390/ani11082380