Infant Exposure to Bisphenol A Can Be Quantitatively Assessed by a Simply Improved High-Performance Liquid Chromatography–Tandem Mass-Spectrometry Method

• Published in: Analytical Sciences Vol. 33; no. 7; pp. 777 - 781
• Main Authors: WANG, Heng, LIU, Liangpo, Syed Ali Musstjab Akber Shah EQANI, SHEN, Heqing
• Format: Journal Article
• Language: English
• Published: Singapore The Japan Society for Analytical Chemistry 2017; Springer Nature Singapore; Japan Science and Technology Agency
• Subjects: Analytical Chemistry; Benzhydryl Compounds - urine; Bisphenol A; Chemistry; Chromatography; Chromatography, High Pressure Liquid; Environmental Exposure; Environmental Pollutants - urine; Exposure; High performance liquid chromatography; HPLC-MS/MS; Humans; Infant; Infant urine; Infants; Ions; Liquid chromatography; Phenols; Phenols - urine; Scientific imaging; Spectrometry; Spectroscopy; Tandem Mass Spectrometry; trace level BPA; Trace levels; Trapping; trapping column; Urine; Infant urine; HPLC-MS/MS; trace level BPA; trapping column
• ISSN: 0910-6340; 1348-2246
• DOI: 10.2116/analsci.33.777

To quantitatively measure trace levels of bisphenol A (BPA) in infant urine, a simply improved high-performance liquid chromatography (HPLC)–tandem mass-spectrometry method was developed and validated. In the present work, a ZORBAX SB-C18 column (2.1 × 30 mm, 3.5 μm) was used for trapping and isolating BPA from the HPLC mobile phase. The result showed that the trapping column can provide effective separation from the background BPA to the sample BPA. This method has a limit of detection (LOD) of 0.048 μg/L, spiked recoveries 85.4% (0.50 – 10.0 μg/L), and relative standard deviations of 1.8 – 15.9%. By using its low-LOD advantage, we firstly reported that Chinese infants (n = 48 with age <6 months) have been ubiquitously exposed to BPA (detection frequency of 93%) with an median level of 0.13 μg/L (ranging from below LOD to 5.04 μg/L).