Food chemistry and biochemistry of enzymatic browning

• Published in: FOOD SCIENCE AND TECHNOLOGY RESEARCH Vol. 28; no. 1; pp. 1 - 12
• Main Author: Murata, Masatsune
• Format: Journal Article
• Language: English
• Published: Tsukuba Japanese Society for Food Science and Technology 2022; The Japanese Society for Food Science and Technology; Japan Science and Technology Agency
• Subjects: Ammonia; Beansprouts; Biochemistry; Cinnamaldehyde; enzymatic browning; Enzymes; Enzymic browning; Heat shock; mild heat shock; PAL; Phenolic compounds; Phenols; Phenylalanine; Phenylalanine ammonia-lyase; Plastids; Polyphenol oxidase; Polyphenols; Substrates; Vacuoles; Wounding
• ISSN: 1344-6606; 1881-3984
• DOI: 10.3136/fstr.FSTR-D-21-00130

Food chemistry and biochemistry of enzymatic browning are reviewed with a focus on the author's research and recent information. Enzymatic browning is categorized into two types, immediate and delayed, according to the time required for browning. Apple browning has been extensively examined as an example of the former. The enzyme polyphenol oxidase (PPO) and its substrate polyphenols are separated into plastids and vacuoles, respectively, in cells. Disruption of this compartmentation by cutting, crushing, wounding, and physiological disorders allows interaction of the enzyme and substrate, and the reaction starts to turn brown. For the immediate type of enzymatic browning, regulation of oxidative polymerization of phenolic compounds by PPO is essential to inhibit browning. Browning of cut lettuce and mung bean sprouts are described here as the delayed type, in which phenylalanine ammonia-lyase (PAL) is induced during storage and phenolic compounds are synthesized de novo. The formed phenolic compounds are successively oxidized by PPO to turn brown. Regulation of PAL induction is crucial to inhibit this type of browning. Treatments with mild heat shock and cinnamaldehyde are described here as physical and chemical methods, respectively, to repress PAL induction and browning.