Differentiation of murine B cells induced by chondroitin sulfate B

• Published in: Cellular immunology Vol. 250; no. 1; pp. 14 - 23
• Main Authors: Yoshihara, Ritsuko, Aoyama, Eriko, Kadota, Yusuke, Kawai, Saeko, Goto, Tomomi, Zhong, Ming, Gohda, Eiichi
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 01.11.2007
• Subjects: Animals; B-Lymphocytes - drug effects; B-Lymphocytes - immunology; CD138; Cell Differentiation - drug effects; Cells, Cultured; Chondroitin sulfate B (CSB); Dermatan Sulfate - pharmacology; Differentiation; Dose-Response Relationship, Drug; Enzyme-Linked Immunosorbent Assay; Female; Flow Cytometry; IgM; Immunoglobulin M - metabolism; Interleukin-4 - metabolism; Interleukin-5 - metabolism; Mice; Mice, Inbred BALB C; Murine B cells; Protein kinase C; Protein Kinase C - physiology; Chondroitin sulfate B (CSB); Protein kinase C; CD138; Murine B cells; Differentiation; IgM
• ISSN: 0008-8749; 1090-2163
• DOI: 10.1016/j.cellimm.2007.12.002

A two-step culture system was used to investigate the role of chondroitin sulfate (CS) B, which is mitogenic to B cells, in differentiation of B cells. Mouse spleen B cells were incubated for 3 days with CSB in the presence of interleukin (IL)-4 and IL-5. After washing, the cells were replated at 10 5 viable cells/well and recultured without CSB in the presence of IL-4 and IL-5. CSB dose-dependently increased IgM production, the greatest enhancement being 450%. Dextran sulfate had a similar effect, whereas other glycosaminoglycans, CSA, CSC, heparin and hyaluronic acid, were marginally effective. Treatment of B cells with CSB resulted in increases in the number of IgM-secreting cells and numbers of CD138-positive cells and CD45R/B220-negative cells. CSB-induced IgM production was inhibited by the protein kinase C (PKC) inhibitor GF109203X but not by the phosphatidylinositol 3-kinase (PI3K) inhibitor wortmannin. These results demonstrated that CSB promoted differentiation of B cells in the presence of IL-4 and IL-5 and suggested that PKC but not PI3K is crucial for CSB-induced IgM production.