Myeloid and plasmacytoid dendritic cells in induced sputum after allergen inhalation in subjects with asthma

• Published in: Journal of allergy and clinical immunology Vol. 126; no. 1; pp. 133 - 139
• Main Authors: Dua, Benny, Watson, Richard M., Gauvreau, Gail M., O'Byrne, Paul M.
• Format: Journal Article
• Language: English
• Published: New York, NY Mosby, Inc 01.07.2010; Elsevier; Elsevier Limited
• Subjects: Adolescent; Adult; Allergens - immunology; Allergic asthma; Allergies; Allergy and Immunology; antigen presenting cells; Asthma; Asthma - immunology; Biological and medical sciences; CCR6; CCR7; Chemokines; Chronic obstructive pulmonary disease, asthma; Dendritic Cells - physiology; Female; Forced Expiratory Volume; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Humans; Immunopathology; Ligands; Male; Medical sciences; Middle Aged; Migration; Myeloid Cells - physiology; myeloid dendritic cells; plasmacytoid dendritic cells; Pneumology; Receptors, CCR6 - analysis; Receptors, CCR7 - analysis; Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis; sputum; Sputum - cytology; Sputum - immunology; Allergic asthma; SSC; sputum; myeloid dendritic cells; FACS; CCL; mDC; pDC; plasmacytoid dendritic cells; CCR; APC; MIP; PE; mAbs; CCR7; CCR6; antigen presenting cells; DC; Chemokine receptor; Plasmacytoid dendritic cell; Antigen presenting cell; Myeloid dendritic cell; Side angle light scatter; Dendritic cell; Fluorescence-activated cell sorting; Chemokine ligand; Phycoerythrin; Monoclonal antibodies; Macrophage inflammatory protein; Allergy; CC chemokine; Immunology; Bronchus disease; Obstructive pulmonary disease; Human; Lung disease; Immunopathology; Respiratory disease; Asthma; Inhalation; CCR6 chemokine receptor; CCR7 chemokine receptor; Allergen; Induced sputum
• ISSN: 0091-6749; 1097-6825; 1097-6825
• DOI: 10.1016/j.jaci.2010.04.006

Dendritic cells are professional antigen presenting cells that mediate the response to inhaled allergens. In animal models, the induction and maintenance of allergic airway inflammation is primarily a function of myeloid dendritic cells, whereas the tolerization to inhaled allergens is likely a function of plasmacytoid dendritic cells. To investigate changes in sputum myeloid and plasmacytoid dendritic cells after allergen inhalation in subjects with asthma. Also, the number of myeloid and plasmacytoid dendritic cells expressing both CCR6 and 7 and their chemokine ligands macrophage inflammatory protein (MIP)–3α and 3β were measured in sputum supernatants. Sputum was induced from 12 dual-responder subjects with allergic asthma before and 7 hours, 24 hours, and 72 hours after inhalation of diluent and allergen. Dendritic cells were enumerated via flow cytometry and the chemokines by using ELISAs. The number of sputum myeloid dendritic cells was significantly higher 24 hours after allergen challenge compared with diluent. Similarly, sputum plasmacytoid dendritic cells increased significantly at 24 hours after allergen challenge. Also, a significant increase in CCR6 + myeloid dendritic cells numbers occurred 72 hours after allergen challenge. In contrast, CCR7 + myeloid dendritic cells, as well as the number of CCR6 + and CCR7 + plasmacytoid dendritic cells, were not different between challenges. Finally, allergen challenge increased sputum levels of MIP-3α, but not MIP-3β, compared with baseline. Both myeloid and plasmacytoid dendritic cells increase in the sputum of subjects with asthma after allergen challenge, suggesting that both subsets are involved in the pathogenesis of allergen responses in asthma.