In Vitro Analyses of the Multifocal Effects of Natural Alkaloids Berberine, Matrine, and Tabersonine against the O’nyong-nyong Arthritogenic Alphavirus Infection and Inflammation

O’nyong-nyong virus (ONNV) is a member of the reemerging arthritogenic alphaviruses that cause chronic debilitating polyarthralgia and/or polyarthritis via their tropism for the musculoskeletal system. Thus, the discovery of dual antiviral and anti-inflammatory drugs is a great challenge in this fie...

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Published inPharmaceuticals (Basel, Switzerland) Vol. 16; no. 8; p. 1125
Main Authors Sandenon Seteyen, Anne-Laure, Guiraud, Pascale, Gasque, Philippe, Girard-Valenciennes, Emmanuelle, Sélambarom, Jimmy
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 01.08.2023
MDPI
Subjects
Alkaloids
anti-inflammatory
antiviral
Antiviral agents
Chemical properties
Chemical Sciences
Chikungunya virus
Control
Epidemics
Fibroblasts
Gene expression
Identification and classification
Infections
Inflammation
Interferon
Kinases
Life Sciences
natural products
O’nyong-nyong virus
Proteins
RNA viruses
Testing
Tumor necrosis factor-TNF
Varieties
Viruses
France
Alkaloids
Antiviral
Natural products
Anti-inflammatory
O'nyong-nyong virus
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Summary:O’nyong-nyong virus (ONNV) is a member of the reemerging arthritogenic alphaviruses that cause chronic debilitating polyarthralgia and/or polyarthritis via their tropism for the musculoskeletal system. Thus, the discovery of dual antiviral and anti-inflammatory drugs is a great challenge in this field. We investigated the effects of the common plant-derived alkaloids berberine (isoquinoline), matrine (quinolizidine), and tabersonine (indole) at a non-toxic concentration (10 μM) on a human fibroblast cell line (HS633T) infected by ONNV (MOI 1). Using qRT-PCR analyses, we measured the RNA levels of the gene coding for the viral proteins and for the host cell immune factors. These alkaloids demonstrated multifocal effects by the inhibition of viral replication, as well as the regulation of the type-I interferon antiviral signaling pathway and the inflammatory mediators and pathways. Berberine and tabersonine proved to be the more valuable compounds. The results supported the proposal that these common alkaloids may be useful scaffolds for drug discovery against arthritogenic alphavirus infection.
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ISSN:1424-8247
1424-8247
DOI:10.3390/ph16081125