Pretransplantation recipient regulatory T cell suppressive function predicts delayed and slow graft function after kidney transplantation

• Published in: Transplantation Vol. 98; no. 7; p. 745
• Main Authors: Nguyen, Minh-Tri J P, Fryml, Elise, Sahakian, Sossy K, Liu, Shuqing, Michel, Rene P, Lipman, Mark L, Mucsi, Istvan, Cantarovich, Marcelo, Tchervenkov, Jean I, Paraskevas, Steven
• Format: Journal Article
• Language: English
• Published: United States 15.10.2014
• Subjects: Acute Kidney Injury - etiology; Aged; CD4-Positive T-Lymphocytes - cytology; Cell Proliferation; Delayed Graft Function - immunology; Female; Glomerular Filtration Rate; Graft Rejection; Graft Survival; Humans; Interleukin-2 Receptor alpha Subunit - metabolism; Kidney Transplantation - adverse effects; Male; Middle Aged; Multivariate Analysis; Odds Ratio; Prospective Studies; Regression Analysis; Renal Insufficiency - complications; Renal Insufficiency - surgery; T-Lymphocytes, Regulatory - cytology; T-Lymphocytes, Regulatory - immunology
• ISSN: 1534-6080
• DOI: 10.1097/TP.0000000000000219

Delayed graft function (DGF) and slow graft function (SGF) are a continuous spectrum of ischemia-reperfusion-related acute kidney injury (AKI) that increases the risk for acute rejection and graft loss after kidney transplantation. Regulatory T cells (Tregs) are critical in transplant tolerance and attenuate murine AKI. In this prospective observational cohort study, we evaluated whether pretransplantation peripheral blood recipient Treg frequency and suppressive function are predictors of DGF and SGF after kidney transplantation. Deceased donor kidney transplant recipients (n=53) were divided into AKI (n=37; DGF, n=10; SGF, n=27) and immediate graft function (n=16) groups. Pretransplantation peripheral blood CD4CD25FoxP3 Treg frequency was quantified by flow cytometry. Regulatory T-cell suppressive function was measured by suppression of autologous effector T-cell proliferation by Treg in co-culture. Pretransplantation Treg suppressive function, but not frequency, was decreased in AKI recipients (P<0.01). In univariate and multivariate analyses accounting for the effects of cold ischemic time and donor age, Treg suppressive function discriminated DGF from immediate graft function recipients in multinomial logistic regression (odds ratio, 0.77; P<0.01), accurately predicted AKI in receiver operating characteristic curve (area under the curve, 0.82; P<0.01), and predicted 14-day estimated glomerular filtration rate in linear regression (P<0.01). Our results indicate that recipient peripheral blood Treg suppressive function is a potential independent pretransplantation predictor of DGF and SGF.