Evidence for expression and function of phosphodiesterase type 5 (PDE‐V) in rat resistance arteries

• Published in: British journal of pharmacology Vol. 132; no. 1; pp. 13 - 17
• Main Authors: Sampson, L J, Hinton, J M, Garland, C J
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.01.2001; Nature Publishing
• Subjects: 3',5'-Cyclic-GMP Phosphodiesterases; Animals; Arteries - drug effects; Arteries - enzymology; Arteries - physiology; Biological and medical sciences; Blood vessels and receptors; Blotting, Western; Cardiovascular system; Cyclic Nucleotide Phosphodiesterases, Type 5; diethylamine NONOate; Fundamental and applied biological sciences. Psychology; Immunohistochemistry; In Vitro Techniques; Male; Medical sciences; Mesenteric Arteries - drug effects; Mesenteric Arteries - physiology; mesenteric artery (rat); Muscle Relaxation - drug effects; Muscle, Smooth, Vascular - drug effects; nitric oxide; Nitric Oxide Donors - pharmacology; Pharmacology. Drug treatments; Phosphodiesterase type V; Phosphoric Diester Hydrolases - biosynthesis; Phosphoric Diester Hydrolases - physiology; Precipitin Tests; Rats; Rats, Sprague-Dawley; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - biosynthesis; Special Reports; Vascular Resistance - drug effects; Vascular Resistance - physiology; Vasodilator agents. Cerebral vasodilators; Vertebrates: cardiovascular system; Vasodilator agent; Rat; Enzyme; 3',5'-Cyclic-GMP phosphodiesterase; Gene product; Rodentia; Contractility; Enzyme inhibitor; Smooth muscle; Esterases; Phosphoric diester hydrolases; Gene expression; In vitro; Biological activity; Protein; Endothelium; Vertebrata; Mesenteric artery; Mammalia; Animal; Nitric oxide; Genetics; Hydrolases; Circulatory system
• ISSN: 0007-1188; 1476-5381
• DOI: 10.1038/sj.bjp.0703831

Evidence is provided for expression and a functional role for phosphodiesterase type V (PDE‐V) in the rat isolated small mesenteric artery. The reverse transcription polymerase chain reaction (RT – PCR) demonstrated mRNA for PDE‐V, while Western blotting and immunocytochemical studies showed corresponding protein expression. Smooth muscle relaxation to the nitric oxide donor, diethylamine NONOate (DEA NONOate; 1 nM – 10 μM; pEC50=6.7±0.3) was potentiated significantly by the specific inhibitor of PDE‐V, 4‐[[3,4‐(methylenedioxy)benzyl]amino]‐6‐chloroquinazoline (MBCQ; 1 μM; pEC50=10.5±0.04). These data show that PDE‐V is expressed in both the smooth muscle and endothelial cells of a resistance artery, and the enzyme can significantly influence nitric oxide‐evoked vasorelaxation. British Journal of Pharmacology (2001) 132, 13–17; doi:10.1038/sj.bjp.0703831