Bi-Enzyme Sensor for Phenolic Compounds with Fluorescent Read-Out

• Published in: Chemistry : a European journal Vol. 19; no. 44; pp. 14977 - 14982
• Main Authors: Strianese, Maria, Zauner, Gerhild, Tabares, Leandro C, Tepper, Armand W. J. W., De Martino, Franco, Pellecchia, Claudio, Aartsma, Thijs J., Canters, Gerard W.
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 25.10.2013; WILEY‐VCH Verlag; Wiley Subscription Services, Inc
• Subjects: Antibiotics; Biosensing Techniques; biosensors; Chemistry; Electrochemistry; Energy transfer; enzymes; Enzymes, Immobilized; Fluorescence; FRET; Glucose; Glucose Dehydrogenases - chemistry; Hydrogen-Ion Concentration; Instrumentation; metalloproteins; Metalloproteins - chemistry; Monophenol Monooxygenase - chemistry; Phenols; Phenols - chemistry; Tyrosinase; enzymes; FRET; biosensors; metalloproteins
• ISSN: 0947-6539; 1521-3765
• DOI: 10.1002/chem.201301876

In this paper, the use of tyrosinase (Ty) from Streptomyces antibioticus, labeled with a fluorescent tag, in combination with soluble quinoprotein (PQQ‐containing) glucose dehydrogenase (s‐GDH) to measure trace amounts of phenols is explored. Proof of concept is provided by a series of experiments, which show a clear quantitative dependence of the response on the phenol concentration. One of the advantages of the detection system is that apart from a standard fluorimeter no further instrumentation is required. Highly amplified detection of phenols: A new biosensor for the detection of trace amounts of phenols in aqueous solution that is based on the use of fluorescently labeled tyrosinase (Ty) and soluble glucose dehydrogenase (s‐GDH) is presented (see figure; TBC=4‐tert‐butyl catechol). The detection is based on a Förster resonant energy transfer mechanism. The only instrumentation required is a simple fluorimeter.