Towards Maintenance-Free Biosensors for Hundreds of Bind/Release Cycles
A single aptamer bioreceptor layer was formed using a common streptavidin–biotin immobilization strategy and employed for 100–365 bind/release cycles. Chemically induced aptamer unfolding and release of its bound target was accomplished using alkaline solutions with high salt concentrations or deion...
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Published in | Angewandte Chemie (International ed.) Vol. 54; no. 7; pp. 2174 - 2178 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Weinheim
WILEY-VCH Verlag
09.02.2015
WILEY‐VCH Verlag Wiley Subscription Services, Inc |
Edition | International ed. in English |
Subjects | |
Online Access | Get full text |
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Summary: | A single aptamer bioreceptor layer was formed using a common streptavidin–biotin immobilization strategy and employed for 100–365 bind/release cycles. Chemically induced aptamer unfolding and release of its bound target was accomplished using alkaline solutions with high salt concentrations or deionized (DI) water. The use of DI water scavenged from the ambient atmosphere represents a first step towards maintenance‐free biosensors that do not require the storage of liquid reagents. The aptamer binding affinity was determined by surface plasmon resonance and found to be almost constant over 100–365 bind/release cycles with a variation of less than 5 % relative standard deviation. This reversible operation of biosensors based on immobilized aptamers without storage of liquid reagents introduces a conceptually new perspective in biosensing. Such new biosensing capability will be important for distributed sensor networks, sensors in resource‐limited settings, and wearable sensor applications.
Reversible biosensors based on a single immobilized aptamer layer were developed and employed for 100–365 bind/release cycles with less than 5 % variation in the binding affinity. These biosensors that operate for multiple sensing cycles may find applications in distributed sensor networks, in resource‐limited settings, and as wearable sensors. |
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Bibliography: | This work has been supported by the DHS (HSHQDS-10-C-00206). We thank J. Burczak for fruitful discussions. ark:/67375/WNG-PGLQZ3ZZ-T DHS - No. HSHQDS-10-C-00206 ArticleID:ANIE201411094 istex:377AD804B5C0A903B6BA17BE76295C2CA9F9103A This work has been supported by the DHS (HSHQDS‐10‐C‐00206). We thank J. Burczak for fruitful discussions. ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1433-7851 1521-3773 |
DOI: | 10.1002/anie.201411094 |