Nucleotide sequence of the Jaagsiekte retrovirus, and exogenous and endogenous type D and B retrovirus of sheep and goats

• Published in: Journal of Virology Vol. 66; no. 8; pp. 4930 - 4939
• Main Authors: York, D.F. (Veterinary Research Institute, Onderstepoort, South Africa), Vigne, R, Verwoerd, D.W, Querat, G
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.08.1992
• Subjects: Animals; Base Sequence; Biological and medical sciences; CAPRIN; CAPRINOS; Codon - genetics; DNA, Viral - genetics; Fundamental and applied biological sciences. Psychology; Genetics; Genome, Viral; Goats - microbiology; Humans; jaagsiekte virus; Lung - microbiology; Microbiology; Molecular Sequence Data; NUCLEOTIDE; NUCLEOTIDOS; Oligodeoxyribonucleotides; Open Reading Frames; OVIN; OVINOS; Phylogeny; Polymerase Chain Reaction - methods; Reading Frames; Repetitive Sequences, Nucleic Acid; Restriction Mapping; RETROVIRIDAE; Retroviridae - genetics; Retroviridae - isolation & purification; RNA, Viral - genetics; Sequence Homology, Nucleic Acid; Sheep - microbiology; Viral Proteins - genetics; Virology; Genome organization; Nucleotide sequence; Retroviridae; Homology; Phylogeny; Virus; Vertebrata; Mammalia; Isolate; Sheep; Goat; Artiodactyla; Molecular cloning; Genome; Ungulata; Aminoacid sequence
• ISSN: 0022-538X; 1098-5514
• DOI: 10.1128/jvi.66.8.4930-4939.1992

The complete genome of the jaagsiekte sheep retrovirus (JSRV), the suspected etiological agent of ovine pulmonary carcinoma, has been cloned from viral particles secreted in lung exudates of affected animals and sequenced. The genome is 7,462 nucleotides long and exhibits a genetic organization characteristic of the type B and D oncoviruses. Comparison of the amino acid sequences of JSRV proteins with those of other retrovirus proteins and phylogenetic studies suggest that JSRV diverged from its type B and D lineage after the type B mouse mammary tumor virus but before the type D oncoviruses captured the env gene of a reticuloendotheliosislike virus. Southern blot studies show that closely related sequences are present in sheep and goat normal genomic DNA, indicating that JSRV could be endogenous in ovine and caprine species