Substrate-induced regulation of the human colonic monocarboxylate transporter, MCT1
Butyrate is the principal source of energy for colonic epithelial cells, and has profound effects on their proliferation, differentiation and apoptosis. Transport of butyrate across the colonocyte luminal membrane is mediated by the monocarboxylate transporter 1 (MCT1). We have examined the regulati...
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Published in | The Journal of physiology Vol. 539; no. 2; pp. 361 - 371 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Oxford, UK
The Physiological Society
01.03.2002
Blackwell Publishing Ltd Blackwell Science Inc |
Subjects | |
Online Access | Get full text |
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Summary: | Butyrate is the principal source of energy for colonic epithelial cells, and has profound effects on their proliferation,
differentiation and apoptosis. Transport of butyrate across the colonocyte luminal membrane is mediated by the monocarboxylate
transporter 1 (MCT1). We have examined the regulation of expression of human colonic MCT1 by butyrate, in cultured colonic
epithelial cells (AA/C1). Treatment with sodium butyrate (NaBut) resulted in a concentration- and time-dependent upregulation
of both MCT1 mRNA and protein. At 2 m m butyrate, the magnitude of induction of mRNA (5.7-fold) entirely accounted for the 5.2-fold increase in protein abundance,
and was mediated by both activation of transcription and enhanced mRNA stability. The other monocarboxylates found naturally
in the colon, acetate and propionate, had no effect. The properties of butyrate uptake by AA/C1 cells were characteristic
of MCT1. Induction of the MCT1 protein resulted in a corresponding increase in the maximal rate of butyrate transport. The
V max for uptake of [U- 14 C]butyrate was increased 5-fold following pre-incubation with 2 m m NaBut, with no significant change in the apparent K m . In conclusion, this study is the first to show substrate-induced regulation of human colonic MCT1. The basis of this regulation
is a butyrate-induced increase in MCT1 mRNA abundance, resulting from the dual control of MCT1 gene transcription and stability
of the MCT1 transcript. We suggest that butyrate-induced increases in the expression and resulting activity of MCT1 serve
as a mechanism to maximise intracellular availability of butyrate, to act both as a source of energy and to influence processes
maintaining cellular homeostasis in the colonic epithelium. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0022-3751 1469-7793 |
DOI: | 10.1113/jphysiol.2001.014241 |