Determination of Pathogenicity and Crude Toxin Activity of Rhizoctonia solani with Rice Tissue In Vitro

[ Objectives] As a traditional inoculation method of rice sheath blight, rice relative lesion height method needs rice grow to late tillering or heading stage and then inoculate in vivo, which is time-consuming and is difficult to control the testing conditions, so it is urgent to search a better al...

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Published inPlant Diseases and Pests(植物病虫害研究：英文版) Vol. 8; no. 3; pp. 6 - 11
Main Authors Shaofeng, Ren, Wenhan, Dong, Lie, Liu, Rui, Yue, Chengyun, Li, Genhua, Yang
Format Journal Article
LanguageEnglish
Published Cranston Wu Chu (USA-China) Science and Culture Media Corporation 01.06.2017
Subjects
Biodiversity
Cotton
Infections
Laboratories
Life sciences
Methods
Pathogenicity
Pathogens
Rhizoctonia
Rice
Studies
体外测定
接种方法
水稻纹枯病
活性
离体叶片
粗毒素
致病性测定
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Summary:[ Objectives] As a traditional inoculation method of rice sheath blight, rice relative lesion height method needs rice grow to late tillering or heading stage and then inoculate in vivo, which is time-consuming and is difficult to control the testing conditions, so it is urgent to search a better alternative method. [ Methods] With rice relative lesion height method （ in vivo inoculation） as the control, the pathogenicity and crude toxin activity of Rhizoctonia solani were determined by rice detached leaf sheath method and rice detached leaf method, to compare the correlation of in vivo and in vitro inoculation. [Results] Rice detached leaf method and rice detached leaf sheath method had significantly positive correlation with rice relative lesion height method, and the determination results of rice detached leaf method and rice detached leaf sheath method could reflect the pathogenicity and crude toxin activity of R. solani accurately. [ Conclusions] Rice detached leaf method and rice detached leaf sheath method are easy to operate and control testing conditions, and could be used as alternative methods to determine pathogenicity and etude toxin activity of R. solani.
Bibliography:Rhizoctonia solani; Pathogenicity; Toxin activity
[ Objectives] As a traditional inoculation method of rice sheath blight, rice relative lesion height method needs rice grow to late tillering or heading stage and then inoculate in vivo, which is time-consuming and is difficult to control the testing conditions, so it is urgent to search a better alternative method. [ Methods] With rice relative lesion height method （ in vivo inoculation） as the control, the pathogenicity and crude toxin activity of Rhizoctonia solani were determined by rice detached leaf sheath method and rice detached leaf method, to compare the correlation of in vivo and in vitro inoculation. [Results] Rice detached leaf method and rice detached leaf sheath method had significantly positive correlation with rice relative lesion height method, and the determination results of rice detached leaf method and rice detached leaf sheath method could reflect the pathogenicity and crude toxin activity of R. solani accurately. [ Conclusions] Rice detached leaf method and rice detached leaf sheath method are easy to operate and control testing conditions, and could be used as alternative methods to determine pathogenicity and etude toxin activity of R. solani.
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ISSN:2152-3932