Isolation and characterization of 2-butoxyethanol degrading bacterial strains
A total of 11 bacterial strains capable of completely degrading 2-butoxyethanol (2-BE) were isolated from forest soil, a biotrickling filter, a bioscrubber, and activated sludge, and identified by 16S rRNA gene sequence analysis. Eight of these strains belong to the genus Pseudomonas ; the remaining...
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Published in | Biodegradation (Dordrecht) Vol. 31; no. 3; pp. 153 - 169 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Dordrecht
Springer Netherlands
01.06.2020
Springer Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | A total of 11 bacterial strains capable of completely degrading 2-butoxyethanol (2-BE) were isolated from forest soil, a biotrickling filter, a bioscrubber, and activated sludge, and identified by 16S rRNA gene sequence analysis. Eight of these strains belong to the genus
Pseudomonas
; the remaining three strains are
Hydrogenophaga pseudoflava
BOE3,
Gordonia terrae
BOE5, and
Cupriavidus oxalaticus
BOE300. In addition to 2-BE, all isolated strains were able to grow on 2-ethoxyethanol and 2-propoxyethanol, ethanol,
n
-hexanol, ethyl acetate, 2-butoxyacetic acid (2-BAA), glyoxylic acid, and
n
-butanol. Apart from the only gram-positive strain isolated, BOE5, none of the strains were able to grow on the nonpolar ethers diethyl ether, di-
n
-butyl ether,
n
-butyl vinyl ether, and dibenzyl ether, as well as on 1-butoxy-2-propanol. Strains
H. pseudoflava
BOE3 and two of the isolated pseudomonads,
Pseudomonas putida
BOE100 and
P. vancouverensis
BOE200, were studied in more detail. The maximum growth rates of strains BOE3, BOE100, and BOE200 at 30 °C were 0.204 h
−1
at 4 mM, 0.645 h
−1
at 5 mM, and 0.395 h
−1
at 6 mM 2-BE, respectively. 2-BAA,
n
-butanol, and butanoic acid were detected as potential metabolites during the degradation of 2-BE. These findings indicate that the degradation of 2-BE by the isolated gram-negative strains proceeds via oxidation to 2-BAA with subsequent cleavage of the ether bond yielding glyoxylate and
n
-butanol. Since
Gordonia terrae
BOE5 was the only strain able to degrade nonpolar ethers like diethyl ether, the degradation pathway of 2-BE may be different for this strain. |
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ISSN: | 0923-9820 1572-9729 |
DOI: | 10.1007/s10532-020-09900-3 |