Migration and proliferation of retinal pigment epithelium on extracellular matrix ligands

• Published in: Journal of rehabilitation research and development Vol. 43; no. 6; pp. 713 - 722
• Main Authors: Wang, Hao, Van Patten, Yancy, Sugino, Ilene K, Zarbin, Marco A
• Format: Journal Article
• Language: English
• Published: United States Department of Veterans Affairs 01.09.2006; Superintendent of Documents
• Subjects: Age Factors; Aged; Aged, 80 and over; Carbon dioxide; Care and treatment; Cell migration; Cell Movement; Cell Proliferation; Collagen Type I; Collagen Type IV; Dentistry; Dissection; Extracellular Matrix; Fibronectins; Gene therapy; Humans; Laminin; Macular degeneration; Middle Aged; Patients; Pigment Epithelium of Eye - cytology; Pigment Epithelium of Eye - embryology; Retinal pigment epithelium; Transplants & implants; Wound healing; United States
• ISSN: 0748-7711; 1938-1352
• DOI: 10.1682/jrrd.2005.06.0114

We compared aged adult and fetal retinal pigment epithelium (RPE) migration and proliferation in cell culture. Aged adult RPE resurfaced on dishes coated with human or mouse laminin, human or rat collagen I, human or mouse collagen IV, or human fibronectin. Resurfacing was best on bovine corneal endothelial cell extracellular matrix. Resurfacing was worst on mouse laminin. Fetal RPE resurfacing was robust on all surfaces studied except human and mouse laminin. In general, fetal RPE resurfacing was much greater than that seen with aged RPE on comparable surfaces. The degree of cell proliferation (labeled by Ki-67) was much greater in fetal than adult RPE cells.