Identification and characterization of enhancer agonist human cytotoxic T-cell epitopes of the human papillomavirus type 16 (HPV16) E6/E7

• Published in: Vaccine Vol. 35; no. 19; pp. 2605 - 2611
• Main Authors: Tsang, Kwong Y, Fantini, Massimo, Fernando, Romaine I, Palena, Claudia, David, Justin M, Hodge, James W, Gabitzsch, Elizabeth S, Jones, Frank R, Schlom, Jeffrey
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 02.05.2017; Elsevier Limited
• Subjects: Agonist epitope; Allergy and Immunology; Antigens; Binding; Cancer; Cancer therapies; Cervical cancer; CTLs; Cytotoxicity; Dendritic cells; Epitope Mapping; Epitopes; Epitopes, T-Lymphocyte - immunology; Etiology; Female; Gene expression; Head; Head & neck cancer; Histocompatibility Antigens Class I - metabolism; HPV16; Human papillomavirus; Humans; Identification; Infections; Lymphocytes; Lymphocytes T; Neck; Oncogene Proteins, Viral - immunology; Oncogene Proteins, Viral - metabolism; Papillomavirus E7 Proteins - immunology; Papillomavirus E7 Proteins - metabolism; Peptides; Platforms; Protein Binding; Proteins; Public health; Repressor Proteins - immunology; Repressor Proteins - metabolism; Squamous cell carcinoma; T cell receptors; Vaccines; Viruses; ORF; antigen-presenting cell; E; IVS; CTLs; MHC; open reading frame; major histocompatibility complex; L; early gene; HPV; Agonist epitope; MFI; E6; multiplicity of infection; natural killer; E7; Ad5; GM-CSF; late gene; mean fluorescence intensity; adenovirus serotype 5; human leukocyte antigen; MOI; human papilloma virus; PBMC; HPV16; in vitro stimulation; APC; cytotoxic T lymphocyte; HNSCC; granulocyte-macrophage colony-stimulating factor; CTL; HLA; NK; dendritic cell; peripheral blood mononuclear cell; DC; head and neck squamous cell carcinoma
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2017.03.025

Highlights • Agonist epitopes of HPV have been identified. • T cells generated with HPV agonist epitopes vs. native counterparts demonstrate enhanced tumor cell lysis. • Algorithms and MHC binding do not always predict peptide-directed tumor lysis.