Long-Term Mastication Changed Salivary Metabolomic Profiles

Saliva is an ideal biofluid for monitoring oral and systemic health. Repeated mastication is a typical physical stimulus that improves salivary flow and oral hygiene. Recent metabolomic studies have shown the potential of salivary metabolomic components for various disease monitoring systems. Here,...

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Published inMetabolites Vol. 12; no. 7; p. 660
Main Authors Saeki, Yoji, Takenouchi, Akane, Otani, Etsuyo, Kim, Minji, Aizawa, Yumi, Aita, Yasuko, Tomita, Atsumi, Sugimoto, Masahiro, Matsukubo, Takashi
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 18.07.2022
MDPI
Subjects
Alanine
Biomarkers
Biosynthesis
chewing
Chewing gum
Creatinine
Dental caries
Disease prevention
Glutamine
Hygiene
Liquid chromatography
liquid chromatography–mass spectrometry
Mass spectroscopy
Mastication
Metabolism
Metabolites
metabolomic profile
Metabolomics
Oral hygiene
Saliva
Thermogenesis
saliva
liquid chromatography–mass spectrometry
mastication
metabolomic profile
chewing
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Summary:Saliva is an ideal biofluid for monitoring oral and systemic health. Repeated mastication is a typical physical stimulus that improves salivary flow and oral hygiene. Recent metabolomic studies have shown the potential of salivary metabolomic components for various disease monitoring systems. Here, we evaluated the effect of long-term mastication on salivary metabolomic profiles. Young women with good oral hygiene (20.8 ± 0.3 years, n = 17) participated. They were prohibited from chewing gum during control periods (4 weeks each) and were instructed to chew a piece of gum base seven times a day for 10 min each time during the intervention period. Paired samples of unstimulated whole saliva collected on the last day of the control and intervention period were compared. Liquid chromatography−time-of-flight mass spectrometry successfully quantified 85 metabolites, of which 41 showed significant differences (p < 0.05, Wilcoxon paired test corrected by false discovery rate). Except for a few metabolites, such as citrate, most metabolites showed lower concentrations after the intervention. The pathways related to glycogenic amino acids, such as alanine, arginine, and glutamine, altered considerably. This study suggests that long-term mastication induces unstimulated salivary component-level changes.
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ISSN:2218-1989
2218-1989
DOI:10.3390/metabo12070660