Radiolabeling of cuticular proteins of the brown-winged green bug, Plautia stali Scott (Hemiptera: Pentatomidae)

Fourth-stadium nymphs of the brown-winged green bug, Plautia stali, were orally labeled with [35S]cysteine. We then measured the levels of radioactivity in the fourth- and last-stadia exuvia. Levels of radioactivity in the exuvia increased with increasing doses of [35S]cysteine. The radioactivity le...

Full description

Saved in:
Bibliographic Details
Published inApplied Entomology and Zoology Vol. 37; no. 2; pp. 279 - 283
Main Authors Ihara, Fumio, Mishiro, Koji, Sato, Takeru
Format Journal Article
LanguageEnglish
Published JAPANESE SOCIETY OF APPLIED ENTOMOLOGY AND ZOOLOGY 2002
Subjects
[35S]cysteine
cuticular proteins
Pentatomidae
Plautia stali
radiolabel
Online AccessGet full text

Cover

More Information
Summary:Fourth-stadium nymphs of the brown-winged green bug, Plautia stali, were orally labeled with [35S]cysteine. We then measured the levels of radioactivity in the fourth- and last-stadia exuvia. Levels of radioactivity in the exuvia increased with increasing doses of [35S]cysteine. The radioactivity levels of labeled exuvia treated with KOH suggested that the [35S]cysteine was incorporated into cuticular proteins. The degradation pattern of labeled exuvia by bovine pancreas protease and crude enzyme solutions prepared from entomopathogenic fungi suggested that radiolabeled exuvium can be used as a substrate for cuticle-degrading enzymes.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0003-6862
1347-605X
DOI:10.1303/aez.2002.279