Transcriptome regulation and chromatin occupancy by E2F3 and MYC in mice

• Published in: Scientific data Vol. 3; no. 1; p. 160008
• Main Authors: Tang, Xing, Liu, Huayang, Srivastava, Arunima, Pécot, Thierry, Chen, Zhong, Wang, Qianben, Huang, Kun, Sáenz-Robles, Maria Teresa, Cantalupo, Paul, Pipas, James, Leone, Gustavo
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 16.02.2016; Nature Publishing Group
• Subjects: 631/136/2091; 631/1647/2017/2079; 631/1647/2217/2088; 631/337/572/2102; 631/337/641/1655; Animal genetics; Animals; Binding Sites; Biochemistry, Molecular Biology; Cell Proliferation; Chromatin - genetics; Chromatin - metabolism; Chromatin Immunoprecipitation; Data Descriptor; E2F3 Transcription Factor - genetics; E2F3 Transcription Factor - metabolism; Genes, myc; Genes, Retinoblastoma; Genetics; Humanities and Social Sciences; Intestine, Small - cytology; Intestine, Small - metabolism; Life Sciences; Mice; Molecular biology; multidisciplinary; Oligonucleotide Array Sequence Analysis; Proto-Oncogene Proteins c-myc - genetics; Proto-Oncogene Proteins c-myc - metabolism; Retinoblastoma Protein - genetics; Retinoblastoma Protein - metabolism; Science; Transcriptome; Cell proliferation; Chromatin immunoprecipitation; Transcriptional regulatory elements; Mitosis; Microarray analysis
• ISSN: 2052-4463; 2052-4463
• DOI: 10.1038/sdata.2016.8

E2F3 and MYC are transcription factors that control cellular proliferation. To study their mechanism of action in the context of a regenerating tissue, we isolated both proliferating (crypts) and non-dividing (villi) cells from wild-type and Rb depleted small intestines of mice and performed ChIP-exo-seq (chromatin immunoprecipitation combined with lambda exonuclease digestion followed by high-throughput sequencing). The genome-wide chromatin occupancy of E2F3 and MYC was determined by mapping sequence reads to the genome and predicting preferred binding sites (peaks). Binding sites could be accurately identified within small regions of only 24 bp-28 bp long, highlighting the precision to which binding peaks can be identified by ChIP-exo-seq. Forty randomly selected E2F3- and MYC-specific binding sites were validated by ChIP-PCR. In addition, we also presented gene expression data sets from wild type, Rb-, E2f3- and Myc -depleted crypts and villi within this manuscript. These represent comprehensive and validated datasets that can be integrated to identify putative direct targets of E2F3 and MYC involved in the control of cellular proliferation in normal and Rb -deficient small intestines. Design Type(s) parallel group design • genetic modification design Measurement Type(s) chromatin binding • transcription profiling assay Technology Type(s) transcription factor binding site identification by ChIP-Seq assay • DNA microarray Factor Type(s) Genetic Variation Sample Characteristic(s) Mus musculus • small intestine Machine-accessible metadata file describing the reported data (ISA-Tab format)