Mosquito larvicidal activity of transgenic Anabaena strain PCC 7120 expressing combinations of genes from Bacillus thuringiensis subsp. israelensis

• Published in: Applied and Environmental Microbiology Vol. 63; no. 12; pp. 4971 - 4974
• Main Authors: Wu, X, Vennison, S.J, Liu, H, Ben-Dov, E, Zaritsky, A, Boussiba, S
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.12.1997
• Subjects: Aedes; Aedes aegypti; Anabaena; Anabaena - genetics; Animals; BACILLUS THURINGIENSIS; Bacillus thuringiensis - genetics; Bacillus thuringiensis israelensis; Bacterial Proteins - genetics; Bacterial Proteins - pharmacology; Bacterial Toxins; Bacteriology; Biological and medical sciences; Biological control; Biotechnology; Control; Culicidae; Endotoxins - genetics; Endotoxins - pharmacology; Escherichia coli - genetics; Fundamental and applied biological sciences. Psychology; Gene Expression; GENE TRANSFER; Gene Transfer Techniques; Genes, Bacterial; Genetic engineering; Genetic technics; GENETIC TRANSFORMATION; Genetic Vectors; Genetics; Hemolysin Proteins; INSECTICIDAL PROPERTIES; INSECTICIDAS; INSECTICIDE; INSECTICIDES; Larva; Methods. Procedures. Technologies; Modification of gene expression level; Mosquitoes; Nitrogen; Pest Control, Biological; Phytopathology. Animal pests. Plant and forest protection; Promoter Regions, Genetic; Protozoa. Invertebrates; TRANSFERENCIA DE GENES; TRANSFERT DE GENE; TRANSFORMACION GENETICA; TRANSFORMATION GENETIQUE; Bacillus thuringiensis var. israelensis; Recombinant microorganism; Toxicity; Insecta; Biological control; Bacillaceae; Gene expression; Biological activity; Plasmid; Toxin; Bacillales; Arthropoda; Culicidae; Cyanobacteria; Anabaena; Shuttle vector; Bacteria; Aedes aegypti; Invertebrata; Diptera; Hybrid gene; Microbial insecticide
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/aem.63.12.4971-4974.1997

Various combinations of the genes cryIVA (cry4A), cryIVD (cry11A), and p20 from Bacillus thuringiensis subsp. israelensis were introduced into the nitrogen fixing cyanobacterium Anabaena sp. strain PCC 7120 by means of Escherichia coli-Anabaena shuttle vector pRL488p and were expressed under control of two tandem strong promoters, a cyanobacterial promoter (PpsbA) and an E. coli T7 promoter (PA1). Two of the clones carrying cryIVA plus cryIVD, one with p20 and one without p20, displayed toxicity against third-instar larvae of Aedes aegypti at levels greater than any level previously reported for transgenic cyanobacteria