Physiological and Metabolic Responses of Marine Mussels Exposed to Toxic Cyanobacteria Microcystis aeruginosa and Chrysosporum ovalisporum

• Published in: Toxins Vol. 12; no. 3; p. 196
• Main Authors: Oliveira, Flavio, Diez-Quijada, Leticia, Turkina, Maria V, Morais, João, Felpeto, Aldo Barreiro, Azevedo, Joana, Jos, Angeles, Camean, Ana M, Vasconcelos, Vitor, Martins, José Carlos, Campos, Alexandre
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 20.03.2020; MDPI
• Subjects: Algae; Alkaloids - analysis; Alkaloids - toxicity; Animal species; Animals; Aquatic ecosystems; Aquatic Organisms - metabolism; Aquatic Organisms - microbiology; Bioaccumulation; Bivalvia - metabolism; Bivalvia - microbiology; Coastal ecosystems; Contaminants; Cyanobacteria; Cyanobacteria Toxins; Cylindrospermopsin; Cytoskeleton; Depuration; Ecotoxicology; Environmental changes; Estuaries; Experiments; Exposure; Fresh Water - microbiology; Freshwater organisms; Glycogen; Glycogens; Harmful Algal Bloom; Health aspects; Marine animals; Marine ecosystems; Marine Toxins - analysis; Marine Toxins - toxicity; Metabolic Networks and Pathways - drug effects; Metabolic pathways; Metabolic response; Metabolism; microcystin; Microcystin-LR; Microcystins - analysis; Microcystins - toxicity; Microcystis; Microcystis aeruginosa; Mollusks; Mussels; mytilus galloprovincialis; Pandalus kessleri; Physiology; Poisoning; Protein folding; Proteins; Proteome - metabolism; Proteomics; Rivers; Salinity; Shellfish; shotgun proteomics; Species; toxic cyanobacteria; Toxicity; Toxins; Transcription; Waterways; Portugal; cylindrospermopsin; toxic cyanobacteria; Mytilus galloprovincialis; ecotoxicology; microcystin; shotgun proteomics
• ISSN: 2072-6651; 2072-6651
• DOI: 10.3390/toxins12030196

Toxic cyanobacterial blooms are a major contaminant in inland aquatic ecosystems. Furthermore, toxic blooms are carried downstream by rivers and waterways to estuarine and coastal ecosystems. Concerning marine and estuarine animal species, very little is known about how these species are affected by the exposure to freshwater cyanobacteria and cyanotoxins. So far, most of the knowledge has been gathered from freshwater bivalve molluscs. This work aimed to infer the sensitivity of the marine mussel to single as well as mixed toxic cyanobacterial cultures and the underlying molecular responses mediated by toxic cyanobacteria. For this purpose, a mussel exposure experiment was outlined with two toxic cyanobacteria species, and at 1 × 10 cells/mL, resembling a natural cyanobacteria bloom. The estimated amount of toxins produced by and were respectively 0.023 pg/cell of microcystin-LR (MC-LR) and 7.854 pg/cell of cylindrospermopsin (CYN). After 15 days of exposure to single and mixed cyanobacteria, a depuration phase followed, during which mussels were fed only non-toxic microalga . The results showed that the marine mussel is able to filter toxic cyanobacteria at a rate equal or higher than the non-toxic microalga . Filtration rates observed after 15 days of feeding toxic microalgae were 1773.04 mL/ind.h (for ), 2151.83 mL/ind.h (for ), 1673.29 mL/ind.h (for the mixture of the 2 cyanobacteria) and 2539.25 mL/ind.h (for the non-toxic ). Filtering toxic microalgae in combination resulted in the accumulation of 14.17 ng/g dw MC-LR and 92.08 ng/g dw CYN. Other physiological and biochemical endpoints (dry weight, byssus production, total protein and glycogen) measured in this work did not change significantly in the groups exposed to toxic cyanobacteria with regard to control group, suggesting that mussels were not affected with the toxic microalgae. Nevertheless, proteomics revealed changes in metabolism of mussels related to diet, specially evident in those fed on combined cyanobacteria. Changes in metabolic pathways related with protein folding and stabilization, cytoskeleton structure, and gene transcription/translation were observed after exposure and feeding toxic cyanobacteria. These changes occur in vital metabolic processes and may contribute to protect mussels from toxic effects of the toxins MC-LR and CYN.