Diversity of Bacteria and Glycosyl Hydrolase Family 48 Genes in Cellulolytic Consortia Enriched from Thermophilic Biocompost

• Published in: Applied and Environmental Microbiology Vol. 76; no. 11; pp. 3545 - 3553
• Main Authors: Izquierdo, Javier A, Sizova, Maria V, Lynd, Lee R
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.06.2010; American Society for Microbiology (ASM)
• Subjects: Bacteria; Bacterial Proteins - genetics; Bacteriology; Biodiversity; Biological and medical sciences; Biotechnology; Cellulose; Cellulose - metabolism; Clostridium; Cluster Analysis; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Fundamental and applied biological sciences. Psychology; Gene expression; Glycoside Hydrolases - genetics; Gram-Positive Bacteria - classification; Gram-Positive Bacteria - enzymology; Gram-Positive Bacteria - genetics; Gram-Positive Bacteria - isolation & purification; Microbiology; Molecular Sequence Data; Phylogeny; Ribonucleic acid; RNA; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Soil; Soil Microbiology; Bacteria; Hydrolases; Thermophily; Gene; Enzyme; Diversity
• ISSN: 0099-2240; 1098-5336; 1098-6596
• DOI: 10.1128/AEM.02689-09

The enrichment from nature of novel microbial communities with high cellulolytic activity is useful in the identification of novel organisms and novel functions that enhance the fundamental understanding of microbial cellulose degradation. In this work we identify predominant organisms in three cellulolytic enrichment cultures with thermophilic compost as an inoculum. Community structure based on 16S rRNA gene clone libraries featured extensive representation of clostridia from cluster III, with minor representation of clostridial clusters I and XIV and a novel Lutispora species cluster. Our studies reveal different levels of 16S rRNA gene diversity, ranging from 3 to 18 operational taxonomic units (OTUs), as well as variability in community membership across the three enrichment cultures. By comparison, glycosyl hydrolase family 48 (GHF48) diversity analyses revealed a narrower breadth of novel clostridial genes associated with cultured and uncultured cellulose degraders. The novel GHF48 genes identified in this study were related to the novel clostridia Clostridium straminisolvens and Clostridium clariflavum, with one cluster sharing as little as 73% sequence similarity with the closest known relative. In all, 14 new GHF48 gene sequences were added to the known diversity of 35 genes from cultured species.