Effect of Temperature on the Nanomechanics of Lipid Bilayers Studied by Force Spectroscopy

• Published in: Biophysical journal Vol. 89; no. 6; pp. 4261 - 4274
• Main Authors: Garcia-Manyes, Sergi, Oncins, Gerard, Sanz, Fausto
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.12.2005; Biophysical Society
• Subjects: 1,2-Dipalmitoylphosphatidylcholine - analysis; 1,2-Dipalmitoylphosphatidylcholine - chemistry; Elasticity; Lipid Bilayers - chemistry; Lipids; Measurement; Mechanical properties; Mechanics; Membrane Fluidity; Membranes; Microscopy, Atomic Force - methods; Molecular Conformation; Nanostructures - analysis; Nanostructures - chemistry; Nanostructures - ultrastructure; Phase Transition; Physical properties; Simulation; Single crystals; Spectroscopy, Imaging, Other Techniques; Spectrum analysis; Stress, Mechanical; Studies; Surfactants; Temperature; Temperature effects
• ISSN: 0006-3495; 1542-0086
• DOI: 10.1529/biophysj.105.065581

The effect of temperature on the nanomechanical response of supported lipid bilayers has been studied by force spectroscopy with atomic force microscopy. We have experimentally proved that the force needed to puncture the lipid bilayer ( F y) is temperature dependent. The quantitative measurement of the evolution of F y with temperature has been related to the structural changes that the surface undergoes as observed through atomic force microscopy images. These studies were carried out with three different phosphatidylcholine bilayers with different main phase transition temperature ( T M), namely, 1,2-dimyristoyl- sn-glycero-3-phosphocholine, 1,2-dipalmitoyl- sn-glycero-3-phosphocholine, and 2-dilauroyl- sn-glycero-3-phosphocholine. The solid-like phase shows a much higher F y than the liquid-like phase, which also exhibits a jump in the force curve. Within the solid-like phase, F y decreases as temperature is increased and suddenly drops as it approaches T M. Interestingly, a “well” in the F y versus temperature plot occurs around T M, thus proving an “anomalous mechanical softening” around T M. Such mechanical softening has been predicted by experimental techniques and also by molecular dynamics simulations and interpreted in terms of water ordering around the phospholipid headgroups. Ion binding has been demonstrated to increase F y, and its influence on both solid and liquid phases has also been discussed.