Stalk Phase Formation: Effects of Dehydration and Saddle Splay Modulus

• Published in: Biophysical journal Vol. 87; no. 4; pp. 2508 - 2521
• Main Authors: Kozlovsky, Yonathan, Efrat, Avishay, Siegel, David A., Kozlov, Michael M.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.10.2004; Biophysical Society
• Subjects: Biophysics; Cell Surface Extensions - chemistry; Computer Simulation; Dehydration; Desiccation; Elasticity; Energy Transfer; Finite element analysis; Lipid Bilayers - chemistry; Lipids; Macromolecular Substances - chemistry; Membrane Fluidity; Membrane Fusion; Membranes; Models, Chemical; Models, Molecular; Molecular Conformation; Phase Transition; Phosphatidylcholines - chemistry; Phosphatidylethanolamines - chemistry; Phospholipids - chemistry; Stress, Mechanical; Surface Tension; Water - chemistry
• ISSN: 0006-3495; 1542-0086
• DOI: 10.1529/biophysj.103.038075

One of the earliest lipid intermediates forming in the course of membrane fusion is the lipid stalk. Although many aspects of the stalk hypothesis were elaborated theoretically and confirmed by experiments it remained unresolved whether stalk formation is always an energy consuming process or if there are conditions where the stalks are energetically favorable and form spontaneously resulting in an equilibrium stalk phase. Motivated by a recent breakthrough experiments we analyze the physical factors determining the spontaneous stalk formation. We show that this process can be driven by interplay between two factors: the elastic energy of lipid monolayers including a contribution of the saddle splay deformation and the energy of hydration repulsion acting between apposing membranes. We analyze the dependence of stalk formation on the saddle splay (Gaussian) modulus of the lipid monolayers and estimate the values of this modulus based on the experimentally established phase boundary between the lamellar and the stalk phases. We suggest that fusion proteins can induce stalk formation just by bringing the membranes into close contact, and accumulating, at least locally, a sufficiently large energy of the hydration repulsion.