Heterologous expression of Bartonella adhesin A in Escherichia coli by exchange of trimeric autotransporter adhesin domains results in enhanced adhesion properties and a pathogenic phenotype

• Published in: Journal of bacteriology Vol. 196; no. 12; pp. 2155 - 2165
• Main Authors: Schmidgen, Thomas, Kaiser, Patrick O, Ballhorn, Wibke, Franz, Bettina, Göttig, Stephan, Linke, Dirk, Kempf, Volkhard A J
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 01.06.2014
• Subjects: Adhesins, Bacterial - genetics; Adhesins, Bacterial - metabolism; Animals; Bacterial Adhesion - physiology; Bacterial proteins; Bacteriology; Bartonella; Bartonella henselae; Bartonella henselae - metabolism; Binding sites; Cell adhesion & migration; Cloning, Molecular; Collagen - chemistry; Collagen - metabolism; E coli; Escherichia coli; Escherichia coli - metabolism; Escherichia coli - pathogenicity; Galleria mellonella; Gene expression; Gene Expression Regulation, Bacterial - physiology; Genotype & phenotype; Gram-negative bacteria; Larva - microbiology; Moths - microbiology; Mutation; Protein Structure, Tertiary; Recombinant Proteins; Yersinia
• ISSN: 0021-9193; 1098-5530
• DOI: 10.1128/jb.01461-13

Human-pathogenic Bartonella henselae causes cat scratch disease and vasculoproliferative disorders. An important pathogenicity factor of B. henselae is the trimeric autotransporter adhesin (TAA) Bartonella adhesin A (BadA), which is modularly constructed, consisting of a head, a long and repetitive neck-stalk module, and a membrane anchor. BadA is involved in bacterial autoagglutination, binding to extracellular matrix proteins and host cells, and in proangiogenic reprogramming. The slow growth of B. henselae and limited tools for genetic manipulation are obstacles for detailed examination of BadA and its domains. Here, we established a recombinant expression system for BadA mutants in Escherichia coli allowing functional analysis of particular BadA domains. Using a BadA mutant lacking 21 neck-stalk repeats (BadA HN23), the BadA HN23 signal sequence was exchanged with that of E. coli OmpA, and the BadA membrane anchor was additionally replaced with that of Yersinia adhesin A (YadA). Constructs were cloned in E. coli, and hybrid protein expression was detected by immunoblotting, fluorescence microscopy, and flow cytometry. Functional analysis revealed that BadA hybrid proteins mediate autoagglutination and binding to collagen and endothelial cells. In vivo, expression of this BadA construct correlated with higher pathogenicity of E. coli in a Galleria mellonella infection model.